Fluorescent quantitative detection kit of H1N1 influenza virus A and detection method thereof

A technology for influenza virus and fluorescence quantification, applied in the direction of fluorescence/phosphorescence, microbiological-based methods, biochemical equipment and methods, etc., can solve the problems of increasing biosafety risks by detecting positive references, and achieve highly specific effects
CN101649356AActive Publication Date: 2010-02-17ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
Publication Date
2010-02-17

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Abstract

The invention provides a nucleic-acid fluorescent quantitative RT-PCR detection kit of H1N1 influenza virus A and a detection method thereof. The detection kit mainly comprises a specific primer, a fluorescent probe, a PCR buffer solution, a deoxidized nucleoside triphosphate compound, a DNA polymerase, a reverse transcriptase and an RNA enzyme inhibitor, wherein the specific primer and the fluorescent probe have the following sequences: an upstream primer H1N1 A-FP:5'-AGGTTTGAGATATTCCCCAAGACA-3'; a downstream primer H1N1 A-RP:5'-AATTTTTGTAGAAGCTTTTTGCTCC-3'; and a specific probe H1N1 A-P:5'-F-CATGGCCCAATCATGACTCGAACA-Q-3', wherein F is a fluorescent reporter group; and Q is a fluorescent quenching group. The invention has the advantages that the nucleic-acid fluorescent quantitative RT-PCR detection kit of the H1N1 influenza virus A and the detection method thereof can be applied to the emergent detection and the monitoring of a lab where the H1N1 influenza virus A causes an outbreakepidemic.
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Description

(1) Technical field

[0001] The invention relates to a nucleic acid fluorescent quantitative RT-PCR detection kit and a detection method of type A (H1N1) influenza virus, which can be applied to laboratory emergency detection of outbreaks caused by type A (H1N1) influenza virus. (2) Background technology

[0002] Since March 18, 2009, there has been an outbreak of human infection with H1N1 influenza virus in Mexico. Since the first death case of human infection with influenza A H1N1 virus appeared in Mexico on April 13, 2009, as of May 5, 21 countries around the world have confirmed the existence of influenza A (H1N1) influenza cases through laboratory testing. The number of cases rose to 1,124, including 26 deaths. In addition to Mexico and the United States, many countries have reported confirmed cases of influenza A (H1N1). To this end, the World Health Organization (WHO) raised the global influenza pandemic warning level to level 4, and further raised it to level 5 on A...

Claims

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