Chitosan-collagen-diprospan slow releasing system, preparation method and application thereof
A technology of chitosan and collagen, applied in the field of biotechnology and polymer medical materials, can solve the problems of large side effects and difficult to control the concentration of local application, and achieve the effect of reducing scars, preventing the adhesion of scars and nerves, and promoting the growth of endothelial cells.
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Embodiment 1
[0039] Take 3% acid-soluble chitosan and 1.5% collagen, mix them at a ratio of 1:9, then gradually add 0.2ml of Diprosone suspension, mix thoroughly for 60 minutes, then gradually drop in 36 % formaldehyde 2ml, pour into the mold after 5 minutes, demould after standing for 1 hour, wash with distilled water, then dry in a freezer vacuum dryer for 12 hours intermittently to obtain chitosan-collagen-Diprolone slow-release film, at 4°C deposit. The thickness of the film is 0.5 mm, and the dried film has a tubular structure with one side open.
Embodiment 2
[0041] Take 3% acid-soluble chitosan and 1.5% collagen, mix them at a ratio of 1:9, then gradually add 0.4ml of Diprosone suspension, mix thoroughly for 60 minutes, then gradually drop in 36 % formaldehyde 2ml, pour into the mold after 5 minutes, demould after standing for 1 hour, wash with distilled water, then dry in a freezer vacuum dryer for 12 hours intermittently to obtain chitosan-collagen-Diprolone slow-release film, at 4°C deposit. The thickness of the film is 0.5 mm, and the dried film has a tubular structure with one side open.
Embodiment 3
[0043] Take 3% acid-soluble chitosan and 1.5% collagen, mix them at a ratio of 1:9, then gradually add 0.6ml of Diprosone suspension, mix thoroughly for 60 minutes, then gradually drop in 36 % formaldehyde 2ml, pour into the mold after 5 minutes, demould after standing for 1 hour, wash with distilled water, then dry in a freezer vacuum dryer for 12 hours intermittently to obtain chitosan-collagen-Diprolone slow-release film, at 4°C deposit. The thickness of the film is 0.5 mm, and the dried film has a tubular structure with one side open.
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