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Immunity-chropadography test paper for detecting Yersinia pestis infection and preparation method thereof

A technology of immunochromatographic test paper and plague, which is applied in the field of biochemistry, can solve the problems of long test time and complicated test methods, and achieve strong specificity

Inactive Publication Date: 2010-03-24
SHANGHAI HAITAI JINXIN BIOMOLECULAR DETECTION TECH
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AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide an immunochromatographic test paper for detecting Yersinia pestis infection and its preparation method. The test paper and its preparation method should solve the technical problems of long test time and complex test methods in the prior art for detecting plague infection

Method used

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Examples

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Embodiment 1

[0008] Embodiment 1 detects the preparation of the immune test paper that plague infects

[0009] 1. Materials

[0010] Chlorauric acid (HAuCl 4 .4H 2 O), analytically pure, Shanghai Reagent No. 1 Factory

[0011] Trisodium citrate (Na 3 C 5 h 5 o 7 .2H 2 O), analytically pure, Beijing Chemical Plant

[0012] Potassium carbonate (K 2 CO 3 ) analytically pure, Beijing Chemical Plant

[0013] Staphylococcus aureus protein A (SPA), purchased from Amersham Pharmacia Biotech company Yersinia pestis strain (introduced from Russia, the strain name is E.V, and is now preserved by the strain bank of the Institute of Microbiology and Epidemiology of the Chinese People's Liberation Army, and the preservation number is 410049 )

[0014] Plague positive serum national standard product, guinea pig, ground squirrel, rabbit and human plague positive and negative sera produced by National Institute of Biological Products, provided by Brucella pestis prevention and control base of C...

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Abstract

The invention provides an immunity-chropadography test paper for detecting Yersinia pestis infection, comprising a layer of cellulose nitrate film, wherein a detection line and a quality control lineare arranged on the cellulose nitrate film, and the detection is Yersinia pestis polysaccharide antigen and the quality control line is antibody capable of being combined with staphylococcus aureus protein A and the cellulose nitrate film is arranged on a water absorption pad and a gold-label pad is arranged on the cellulose nitrate film and and a sample pad is arranged on the gold-label pad The invention also provides a preparation method of the immunity-chropadography test paper for detecting Yersinia pestis infection. Compared with the prior method, the immunity-chropadography test paper for detecting Yersinia pestis infection and preparation method thereof are simpler, more quick and more suitable for clinical and special condition with strong specificity.

Description

Technical field: [0001] The invention relates to the field of biochemistry, in particular to an immunochromatographic test paper, in particular to an immunochromatographic test paper for detecting plague bacterial infection and a preparation method thereof. Background technique: [0002] The laboratory diagnosis of Yersinia plague is mainly etiological diagnosis and serum antibody detection. The etiological diagnosis method is to collect secretions, pus, For blood and sputum samples, inoculate trypticase soybean blood agar medium, incubate at 37°C with 5% CO2 for 72 hours, pick off-white, round colonies, prepare slides and do Gram staining, if they are Gram-negative cocci , continue to carry out biochemical identification and serological tests, and if necessary, carry out independent animal testing. The principle of serum antibody detection is: people, domestic animals and wild animals can have serum antibodies with diagnostic significance after infection with Yersinia pest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
Inventor 葛海鹏
Owner SHANGHAI HAITAI JINXIN BIOMOLECULAR DETECTION TECH
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