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Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same

A technology of blood cells and red blood cells, which is applied in the direction of immunoassay, test sample preparation, biological testing, etc., and can solve problems such as complex calibration operations and measurement result errors

Active Publication Date: 2012-07-04
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difference in the environment where the automatic blood analyzer is placed and the performance of the heater of the automatic blood analyzer, sometimes the measured temperature is less than 20°C
Therefore, when the above-mentioned inflection point is 20° C. or higher, when the measurement result of the automatic blood analyzer is to be corrected according to the measurement temperature, complicated calibration work is required.
As a result, when an automatic blood analyzer measures the MCV of a blood sample, an error in the measurement result due to the measurement temperature occurs.

Method used

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  • Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same
  • Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same
  • Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same

Examples

Experimental program
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Effect test

Embodiment 1

[0067] (Preparation of reagents for blood sample dilution)

[0068] 2.20 g of sodium chloride was added to 1L CELLPACK manufactured by Sysmex Corporation as a reagent for diluting blood samples. In CELLPACK added with sodium chloride, polyoxyethylene oleyl ethers with different hydroxyl values ​​were added to make the concentration reach 0.015% by weight to prepare reagents A to E.

[0069] The hydroxyl values ​​of the polyoxyethylene oleyl ether used to prepare reagents A to E are as follows:

[0070] Reagent A: Polyoxyethylene oleyl ether with a hydroxyl value of 49.0

[0071] Reagent B: Polyoxyethylene oleyl ether with a hydroxyl value of 51.0

[0072] Reagent C: Polyoxyethylene oleyl ether with a hydroxyl value of 53.6

[0073] Reagent D: Polyoxyethylene oleyl ether with a hydroxyl value of 54.8

[0074] Reagent E: Polyoxyethylene oleyl ether with a hydroxyl value of 57.1

[0075] (MCV measurement)

[0076] Dilute three normal fresh blood samples 500 times with reagents A to E to prepa...

Embodiment 2

[0080] (Preparation of reagents for blood sample dilution)

[0081] Add a mixture of polyoxyethylene (20) oleyl ether and polyoxyethylene (16) oleyl ether in a ratio of 1:3 to CELLPACK (II) manufactured by Sysmex Corporation as a reagent for diluting blood samples to make the concentration Reagents for dilution of blood samples with a hydroxyl value of 54 were prepared to reach 0.015% by weight.

[0082] (MCV measurement and calibration of measured values)

[0083] Three normal fresh blood samples were diluted 500 times with a blood sample dilution reagent with a hydroxyl value of 54 to prepare measurement samples. Using the prepared measurement sample, the measurement result of MCV measured at ambient temperature using an automatic blood analyzer XE-2100 (manufactured by Sysmex Corporation) is shown in black squares (■) in FIG. 6.

[0084] An automatic blood analyzer XE-2100C (manufactured by Sysmex Corporation) was used to control the measurement temperature to 23° C. or higher to ...

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Abstract

The invention provides a reagent for diluting a blood sample, comprising water, polyoxyethylene alkyl ether having a hydroxyl value of 52 to 60, and an osmo-regulator for regulating the osmotic pressure of the reagent in the range of 150 to 400 mOsm / kg, as well as a method for measuring the mean corpuscular volume of a blood sample.

Description

Technical field: [0001] The present invention relates to a reagent for diluting a blood sample and a method for measuring the average red blood cell volume (hereinafter sometimes referred to as MCV) using the reagent. Background technique: [0002] An automatic blood analyzer is an instrument that automatically analyzes blood samples. As an item measured by this automatic blood analyzer, there is the MCV of a blood sample. MCV is obtained by dividing the packed volume of red blood cells (hereinafter sometimes referred to as RBC) by the number of red blood cells. The hematocrit value is the percentage of red blood cells in a unit volume of whole blood. Therefore, MCV is a value representing the average volume of red blood cells. MCV in Femtoliter (fL) or 10 -15 L is expressed in units. [0003] Here, a method for determining the RBC number and hematocrit value using an automatic blood analyzer is known as the sheath flow DC detection method. The sheath flow DC detection method ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/38G01N33/487G01N33/50
CPCG01N2015/1236G01N15/1209G01N1/38G01N15/131G01N2015/133Y10T436/101666Y10T436/10
Inventor 森悠丞内桥欣也酒井康裕
Owner SYSMEX CORP