Calcium alginate-chitosan sustained-release microsphere carrying growth hormone and application thereof

A technology of growth hormone and calcium alginate, which is applied to the preparation of microspheres, microcapsule preparations, medical preparations of non-active ingredients, etc., can solve the problems that growth hormone cannot be used for embedding, slow release and protection, so as to enhance the human body Effects on immune function, blood lipid reduction, and narrow particle size distribution

Inactive Publication Date: 2010-04-07
张亦军 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many existing slow-release microspheres with bioaffinity have good embedding, slow-release and protection effects on other drugs, but they cannot be used for embedding, slow-release and protection of growth

Method used

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  • Calcium alginate-chitosan sustained-release microsphere carrying growth hormone and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The somatotropin-loaded calcium alginate chitosan slow-release microspheres of the present embodiment, its main component is somatotropin, calcium alginate, chitosan, adopts following steps to prepare:

[0023] First, accurately measure 10IU / mL growth hormone injection and 15mg / ml sodium alginate solution, mix them uniformly at a volume ratio of 1:4, and put them into a syringe; The aperture is 450um, the negative electrode is connected to the metal conductive ring placed in the gel bath solution, and a pulse electrostatic field is formed between the orifice and the gel bath. The pulse electric field device indicates a voltage of 380V, a pulse frequency of 120Hz, and a pulse width of 2ms; the syringe will The liquid is pushed out, the flow rate of the raw material liquid is 8mL / h, and the inherent viscous force and surface tension of the sodium alginate solution are overcome under the action of the electric field force, so that the mixed solution drops into 20mg / ml CaCl ...

Embodiment 2

[0025] The somatotropin-loaded calcium alginate chitosan slow-release microspheres of the present embodiment, its main component is somatotropin, calcium alginate, chitosan, adopts following steps to prepare:

[0026]First, accurately measure 10IU / mL growth hormone injection and 20mg / ml sodium alginate solution, mix them uniformly at a volume ratio of 1:4, and put them into a syringe; The aperture is 600um, the negative electrode is connected to the metal conductive ring placed in the gel bath solution, and a pulse electrostatic field is formed between the orifice and the gel bath. The pulse electric field device indicates a voltage of 380V, a pulse frequency of 120Hz, and a pulse width of 2ms; the syringe will The liquid is pushed out, the flow rate of the raw material liquid is 25mL / h, and the inherent viscous force and surface tension of the sodium alginate solution are overcome under the action of the electric field force, so that the mixed solution drops into 30mg / ml CaCl ...

Embodiment 3

[0028] The somatotropin-loaded calcium alginate chitosan slow-release microspheres of the present embodiment, its main component is somatotropin, calcium alginate, chitosan, adopts following steps to prepare:

[0029] First, accurately measure 10IU / mL growth hormone injection and 5mg / ml sodium alginate solution, mix them uniformly at a volume ratio of 1:4, and put them into a syringe; The aperture is 500um, the negative electrode is connected to the metal conductive ring placed in the gel bath solution, and a pulse electrostatic field is formed between the aperture and the gel bath. The pulse electric field device indicates a voltage of 380V, a pulse frequency of 120Hz, and a pulse width of 2ms; the syringe will The liquid is pushed out, the flow rate of the raw material liquid is 50mL / h, and the inherent viscous force and surface tension of the sodium alginate solution are overcome under the action of the electric field force, so that the mixed solution drops into 5mg / ml CaCl ...

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Abstract

The invention discloses a calcium alginate-chitosan sustained-release microsphere carrying growth hormone. The sustained-release microsphere comprises the principal components including the growth hormone, the calcium alginate and the chitosan. The sustained-release microsphere can be used for preparing the drugs for fracture healing. The sustained-release microsphere has good sphericity and narrow particle size distribution range and can further sustain the release and absorption of the drugs. The microsphere features simple and efficient shaping process and mild shaping conditions. The growth hormone has high entrapment rate and activity, and organic solvents are unnecessary, etc.

Description

technical field [0001] The invention relates to a slow-release microsphere, especially a gel microsphere of calcium alginate and chitosan loaded with growth hormone, and relates to the application of the microsphere. It belongs to the field of biochemical industry. Background technique [0002] Studies have shown that growth hormone has a certain promoting effect on general fractures; other studies have shown that the secretion level of growth hormone in the body decreases with age and estrogen secretion, and the decrease of growth hormone can lead to abnormal bone metabolism and reduced fracture repair ability . In addition to being closely related to blood supply and mechanical factors, the fracture healing process is also regulated and influenced by endocrine hormones and cell growth factors, among which growth hormone plays a certain role. Growth hormone can act on bone tissue in different ways and ways, and IGFs play an important role in transmission and mediation. IG...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K38/27A61K47/36A61P19/08B01J13/02
Inventor 张亦军赛佳明刘爱芬黄勇张增方
Owner 张亦军
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