Method for distinguishing conventional cotton and hybrid cotton in assistant mode and special kit thereof

A kit and hybrid cotton technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of inconspicuous, difficult to distinguish, and difficult to distinguish the F2 generation of hybrid materials.
CN101696450AActive Publication Date: 2010-04-21INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Publication Date
2010-04-21

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Abstract

The invention discloses a method for distinguishing conventional cotton and hybrid cotton in an assistant mode and a special kit thereof. The method for distinguishing the conventional cotton and the hybrid cotton in the assistant mode comprises the following steps of (1) extracting a genomic DNA of cotton to be distinguished; (2) respectively carrying out PCR amplification on each pair of primers in the kit by using the genomic DNA of the cotton to be distinguished as a template to obtain the spectrums of PCR amplification products of each pair of the primers; and (3) detecting spectrums of the PCR amplification products of each pair of the primers. Proved by experiments, the kit, an SSR spectrum and the distinguishing method can effectively, accurately and quickly distinguish the cotton to be tested to be the conventional cotton or the hybrid cotton and a cotton seed to be tested to be an F1 generation or an F2 generation, thus the kit, the SSR spectrum and the distinguishing method have wide application prospect.
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Description

technical field

[0001] The invention relates to a method for assisting identification of conventional cotton and hybrid cotton and a special kit thereof. Background technique

[0002] In cotton variety approval, cotton regional test is very important. The main purpose of cotton regional test is to identify the high yield, disease resistance, fiber quality, adaptability and stability of cotton varieties in different regions, and provide a basis for the approval and promotion of cotton varieties. scientific basis. The cotton varieties currently participating in the test are divided into conventional cotton and hybrid cotton. Conventional cotton refers to the cotton variety that adopts conventional breeding methods such as systematic breeding, cross breeding, mutation breeding, etc., and undergoes multiple generations of selfing to make the genotype homozygous. Compared with conventional cotton, hybrid cotton is the use of heterosis in cotton. It is a cotton variety whose gen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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