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UDP-glucuronosyltransferase isomerase, coding gene thereof and use thereof

A uridine diphosphate glucose and gene technology, which is applied to uridine diphosphate glucuronide isomerase and its encoding gene and application fields, can solve the problems such as the loss of stomatal opening and closing function of isolated Commelina officinalis leaves, and achieve significant Economic value and application prospects, the effect of increasing fiber length, improving quality and yield

Inactive Publication Date: 2012-05-23
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2003, Jones et al. used arabinanase to hydrolyze pectin RGI to lose the opening and closing function of stomata in isolated Commelina leaves

Method used

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  • UDP-glucuronosyltransferase isomerase, coding gene thereof and use thereof
  • UDP-glucuronosyltransferase isomerase, coding gene thereof and use thereof
  • UDP-glucuronosyltransferase isomerase, coding gene thereof and use thereof

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Embodiment 1

[0049] Embodiment 1, cloning of uridine diphosphate glucuronate isomerase coding gene

[0050] 1. Cloning of GhGAE1 gene

[0051] Select wild-type upland cotton variety Xuzhou 142 (WT) (the national mid-term cotton seed bank of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) fibers 10 days after flowering, extract total RNA, and extract total RNA according to the RNAgents Total RNA Isolation System kit of Promega Company conduct. Take 5g of total RNA and reverse transcribe to obtain one strand of cDNA. The reaction system is as follows:

[0052] Total RNA 10 μL (5 μg), Oligo (dT) (20 μmol / L) 1.5 μL, 10× buffer 2.5 μL, dNTPmix (2.5 mmol / L) 2 μL, sterile water 8 μL.

[0053] Add 1 μL (200U) SuperScript to the reaction system after bathing in water at 42°C for 1 min TM IIRT, mixed gently, incubated at 42°C for 50min; bathed in water at 70°C for 15min, terminated the reaction; obtained the first strand of cDNA.

[0054] Primers were designed for ...

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Abstract

The invention discloses a UDP-glucuronosyltransferase isomerase, a coding gene thereof and use thereof. The UDP-glucuronosyltransferase isomerase is a protein a or a protein b, wherein the protein a has an amino acid sequence represented by the No.2 sequence in a sequence table; and the protein b is derived from the protein a by synthesizing UDP glucuronic acid with amino acid sequence represented by the No.2 sequence in the sequence table, through substituting and / or losing and or adding one or several amino acids. The invention also discloses a coding gene of the protein. The UDP-glucuronosyltransferase isomerase coding gene can be introduced into cotton to lengthen cotton fibers and improve the quality and yield of cotton fibers. The UDP-glucuronosyltransferase isomerase and the codinggene of the invention have great economic values and application prospects.

Description

technical field [0001] The invention relates to uridine diphosphate glucuronate isomerase and its coding gene and application. Background technique [0002] Cotton fiber is a unicellular structure differentiated from the outer skin cells of the ovule. Cotton fiber is an important raw material for the textile industry and has important economic value. Fiber quality determines its final length and strength. At the same time, cotton fiber cells are also an ideal system for studying important biological phenomena such as cell elongation, differentiation and cell wall synthesis. Therefore, the study of fiber elongation has important economic value and theoretical significance. [0003] The development process of cotton fiber cells is a process of supernormal cell elongation and supernormal cell wall thickening. The length of upland cotton is about 3.0cm, and the diameter of the upland cotton cell wall is 11-22μm, that is to say, the aspect ratio is 1000-3000. Cotton fiber ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/90C12N15/61C12N15/63C12N5/10C12N1/00C12P19/30C12N15/82C12R1/00
Inventor 朱玉贤庞朝友逄宇王慧靳翔秦咏梅
Owner PEKING UNIV
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