Composting method for inoculating compound bacterium agent in secondary fermentation stage
A secondary fermentation and compound bacterial agent technology, which is applied in the field of solid waste resource utilization, can solve the problems of low fertilizer efficiency and long composting time, and achieve the effects of shortening composting time, prolonging high temperature, and shortening composting time
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[0017] The composting method for inoculating composite bacterial agents in the secondary fermentation stage of the present invention comprises a compound bacterial agent preparation unit and an inoculated composting unit.
[0018] The compound bacterial agent preparation unit is to collect 10 g of samples in the high temperature period of the secondary fermentation section of the compost, add 100 mL of sterile water for dilution, and put them into a constant temperature incubator with a rotation speed of 150 rpm and a temperature of 55 ° C for 3 hours. Pipette 25-30mL solution into a centrifuge tube, centrifuge at 2000r / min for 5min, take the supernatant into a 250mL Erlenmeyer flask, add the prepared enrichment medium to the 250mL conical flask in a certain proportion Put the Erlenmeyer flask into a constant temperature incubator with a rotation speed of 150 rpm and a temperature of 55°C. Because the enriched medium provides the nutrients needed for the growth of microorganism...
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