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Preparation method and application of Arthrobacter tobacco zl-1 and its compost low-temperature fermentation bacterial agent

A technology of Arthrobacter tobacco and low-temperature fermentation bacteria, which is applied in the biological field and can solve the problems of difficult rapid heating and start-up of piles, and ineffective fermentation of manure.

Active Publication Date: 2022-03-01
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compost is easily affected by the ambient temperature. When the ambient temperature is low, it is difficult for the compost to heat up quickly and start, resulting in the ineffective fermentation of manure.

Method used

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  • Preparation method and application of Arthrobacter tobacco zl-1 and its compost low-temperature fermentation bacterial agent
  • Preparation method and application of Arthrobacter tobacco zl-1 and its compost low-temperature fermentation bacterial agent
  • Preparation method and application of Arthrobacter tobacco zl-1 and its compost low-temperature fermentation bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation, screening and identification of Arthrobacter tobacco ZL-1

[0031] The bacterial strain of the present invention is isolated from the cow dung of Dalian Xinle Dairy Cow Breeding Factory.

[0032] 1. Isolation and screening of Arthrobacter tobacco ZL-1

[0033] (1) Take by weighing 10g of the collected cow dung sample and add it to a conical flask equipped with 90mL PBS buffer solution, and add sterile glass beads (which help the sample to be evenly dispersed in PBS buffer solution), at room temperature, 180rpm Oscillate on a shaking table for 20 minutes, and let stand for 10 minutes to prepare the bacterial suspension (ie 10 -1 Cow manure sample diluent); Then carry out 10-fold serial dilution with PBS buffer solution, obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 , 10 -8 Dilute the suspension, choose 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 100 μL each of the diluted suspension was evenly spread on the beef extract peptone solid medium, an...

Embodiment 2

[0047] The preparation method of Arthrobacter tobacco ZL-1 composting low-temperature fermentation bacterial agent comprises the steps of:

[0048] 1. Preparation of seed solution: Streak the Arthrobacter tobacco ZL-1 strain (CGMCC NO.16246) stored on the solid medium on the slant and transfer it to the solid medium plate, and place it in a constant temperature incubator at 10°C for 72 hours for rejuvenation. The single colony of Arthrobacter tobacco ZL-1 obtained by rejuvenation was inoculated into beef extract peptone liquid medium, and cultured on a shaking table at 15°C for 48 hours to obtain Arthrobacter tobacco ZL-1 seed liquid, and the preparation ratio of beef extract peptone liquid medium was : Each 1000ml contains 3g of beef extract, 10g of peptone, 5g of sodium chloride, the balance is water, pH7.0~7.2, sterilized at 121°C for 20 minutes;

[0049] 2. Preparation of fermented liquid: inoculate the seed liquid into the fermentation medium according to the ratio of 1% ...

Embodiment 3

[0051] Embodiment 3: the effect evaluation of composting low-temperature fermentation bacterial agent application

[0052] 1. At an ambient temperature of 5-10°C, establish an evaluation experiment for the effect of composting low-temperature fermentation bacteria agents. Fully mix fresh cow dung and straw at a mass ratio of 4:1, adjust the water content to about 60%, and obtain a cow dung composting system. Arthrobacter nicotiana ZL-1 compost low-temperature fermentation bacterial agent was inoculated at 0.5% by weight in a cow dung composting system with a length of 1m, a width of 0.8m, and a height of 1.2m, and a blank control group without bacterial agent was set.

[0053] 2. Record the ambient temperature and the temperature of the pile every day. The temperature of the pile is measured by a piercing thermometer. The measurement position is 30cm below the height of the center of the pile. The temperature of the pile is measured once a day at 9:00 and 17:00 and the average...

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Abstract

The invention discloses a strain of Arthrobacter nicotianae ZL-1 and a preparation method of Arthrobacter nicotianae ZL-1 composting low-temperature fermentation bacterial agent, which comprises the steps of: pressing Arthrobacter nicotianae ZL-1 bacterial liquid and corncob powder Volume mass ratio 2:1 (mL / g) mixed evenly, dried to moisture content≤25%, obtain Arthrobacter tobacco ZL-1 compost low-temperature fermentation bacterial agent; Wherein, the Arthrobacter tobacco ZL-1 bacteria liquid The number of live bacteria>1×10 8 CFU / mL. The compost fermentation bacteria agent prepared by the invention can be used alone or in combination with other bacteria agents, aiming at improving the degradation ability of biological macromolecules, improving the fermentation rate of compost and the quality of bio-organic fertilizer. In a low-temperature environment of 5-10°C, the compost heap added with this bacterial agent heats up quickly and has a high temperature. It enters a high-temperature period on the 7th day and lasts for 8-9 days, with a maximum temperature of 63.4°C, which has reached the level of harmless composting in my country. standard.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Arthrobacter tobacco ZL-1 and a method for preparing a compost low-temperature fermentation bacterial agent containing the Arthrobacter tobacco ZL-1. Background technique [0002] Arthrobacter bacteria are a kind of strict aerobic bacteria, which are widely distributed in soil and water environment, and Arthrobacter bacteria are found in the waters of Antarctic and Arctic. Currently, research on the bacteria of the genus Arthritis has focused on the ability to produce amino acids, vitamins, and enzymes. Studies have shown that Arthrobacter tobacco, as an aquatic probiotic, can improve the immunity of aquatic animals by stimulating the immune system of aquatic animals or inhibiting the growth of pathogenic bacteria. In addition, it was reported that some researchers isolated a kind of Arthrobacter tobacco with nitrogen-fixing ability from the soil after composting of garb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C12R1/06
CPCC12N1/20C12R2001/06C12N1/205C05F17/20Y02W30/40
Inventor 李晓宇张喆徐永平陈岩王丽丽侯扶琴陈红莉吴妍妍王思佳
Owner DALIAN UNIV OF TECH
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