Method for searching ALK3 downstream gene related to congenital heart disease-ventricular septal defect
A technology for congenital heart disease and ventricular septal defect, which is applied in the field of searching for genes related to disease-causing diseases, and can solve problems such as difficult screening of downstream genes and unclear organ specificity
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[0026] The present invention is specifically described below by the embodiment, only for further illustrating the present invention, can not be interpreted as the limitation of protection scope of the present invention, the technical engineer of this field can make some non-essential improvements and improvements to the present invention according to the content of the above-mentioned invention Adjustment.
[0027] (1) Obtaining of heart-specific ALK3 gene knockout mice: mating of female α-MHC Cre+ / -, ALK3+ / - and male ALK3F / F (the fragment of Lox P inserted on both sides of the second exon of ALK3 gene) α-MHC Cre+ / -, ALK3F / - and α-MHC Cre+ / -, ALK3F / + mouse embryos can be obtained. Such as figure 1 As shown, wherein, the black rectangle represents the ALK3 gene, the white rectangle represents the knockout of the ALK3 gene, and the black arrow represents the loxP sequence.
[0028] (2), extraction of total RNA and mRNA: adopt total RNA and mRNA kit to extract and purify total ...
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