Method for manufacturing animal model of qi-deficiency and blood-stasis disease and evaluation method thereof

Abstract

The invention provides a method for manufacturing animal model of the qi-deficiency and blood-stasis disease, which particularly comprises the following steps: manufacturing an animal model of qi-deficiency syndrome by exhaustive exercise and / or basic food intake control and / or large dose of propranolol administration; and establishing a cerebral ischemia qi-deficiency and blood-stasis disease model by recovering blood supply of an artery after the qi-deficiency syndrome animal model blocks the artery in the brain.

Description

technical field [0001] The invention relates to a method for making an animal model, in particular to a method for making an animal model of Qi deficiency and blood stasis combined with disease and syndrome and an evaluation method thereof. Background technique [0002] Qi-deficiency and blood stasis syndrome is a common syndrome in many clinical diseases. During the years of objective and standardized research on Qi-deficiency and blood stasis syndrome, predecessors have initially revealed it from microscopic perspectives such as blood rheology, vascular regulatory substances, and histomorphological changes. The biological basis of qi deficiency and blood stasis syndrome. Qi is the most basic substance that constitutes the human body and maintains the life activities of the human body. Qi deficiency is a pathological change caused by insufficient generation and source of qi or excessive consumption of qi, which cannot function normally, resulting in decreased functional ac...

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Problems solved by technology

Problems and Prospects in Making Animal Models of Qi Deficiency and Blood Stasis Syndrome Although people have made continuous explorations in making animal models of Qi Deficiency and Blood Stasis Syndrome, and established a variety of model making methods, these methods are not ideal, and are more or less There are some problems in the etiology simulation using multi-factor modeling (swimming, diet control, panic, cold and dampness, etc.). Poor operability and stability; only etiology simulation without disease simulation and positioning, this model neither conforms to the disease development process, but also makes the objective indicators of detection broad; recreate the disease model on the basis of multi-factor modeling to create a combination of disease and syndrome Animal model, although the production of this model conforms to both the theory of traditional Chinese medicine and clinical practice, but the experimental animals are difficult to withstand excessive blows, the mortality rate is high, and the feasibility of the experiment is poor; the combination of the two models of Chinese and Western medicine may influence each other, It is difficult to meet the requirements of animal models that meet both disease and syndrome; if both disease and syndrome meet the required requirements, the pathophysiological changes in animals may exceed the scope of existing diseases; in addition, model evaluation The system lacks quantitative and standardized macro and micro diagnostic standards

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