Method for manufacturing animal model of qi-deficiency and blood-stasis disease and evaluation method thereof

An animal model and production method technology, which is applied in the fields of drug devices, other medical devices, animal husbandry, etc., can solve the problem that the model evaluation system lacks quantitative and standardized macro and micro diagnostic standards, poor experimental feasibility, and it is difficult to meet the requirements of animal models. And other issues

Inactive Publication Date: 2010-05-19
DONGFANG HOSPITAL BEIJING UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Problems and Prospects in Making Animal Models of Qi Deficiency and Blood Stasis Syndrome Although people have made continuous explorations in making animal models of Qi Deficiency and Blood Stasis Syndrome, and established a variety of model making methods, these methods are not ideal, and are more or less There are some problems in the etiology simulation using multi-factor modeling (swimming, diet control, panic, cold and dampness, etc.). Poor operability and stability; only etiology simulation without disease simulation and positioning, this model neither conforms to the disease development process, but also makes the objective indicators of detection broad; recreate the disease model on the basis of multi-factor modeling to create a combinati

Method used

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  • Method for manufacturing animal model of qi-deficiency and blood-stasis disease and evaluation method thereof
  • Method for manufacturing animal model of qi-deficiency and blood-stasis disease and evaluation method thereof
  • Method for manufacturing animal model of qi-deficiency and blood-stasis disease and evaluation method thereof

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Effect test

Embodiment 1

[0088] Animal grouping: 36 healthy male SD rats with a body weight of (200 ± 20) g were purchased from Weitong Lihua Company, and were randomly divided into 3 groups: blank control group (8 rats), fed normally for 22 days; simple MCAO control group ( 12 rats) were fed for 3 weeks, followed by thread embolization for 2 hours of ischemia and reperfusion for 8 hours; in the compound model group (16 rats), continued exhaustive swimming for 3 weeks, then thread embolization for 2 hours of ischemia and then 8 hours of reperfusion.

[0089] Qi deficiency syndrome modeling method: the normal group does not swim; the compound model group swims adaptively after swimming for 2 days (adaptive swimming is not included in the modeling time), and begins formal continuous swimming for 21 days, bearing weight (8% of body weight) every day Swim once, 5-10 minutes a day, for 3 weeks.

[0090] Swimming conditions:

[0091] Swimming pool: diameter 150cm, height 50cm, water depth 40cm;

[0092] W...

Embodiment 2

[0107] Animal grouping: 36 healthy male SD rats with a body weight of (200 ± 20) g were purchased from Weitong Lihua Company, and were randomly divided into 3 groups: blank control group (8 rats), fed normally for 22 days; simple MCAO control group ( 12 rats) were fed normally for 4 weeks, followed by thread embolization for 4 hours of ischemia and reperfusion for 20 hours; compound model group (16 rats), continued exhaustive swimming for 4 weeks, then thread embolization for 4 hours of ischemia and then 20 hours of reperfusion.

[0108] Qi deficiency syndrome modeling method: the normal group does not swim; the compound model group swims adaptively after swimming for 2 days (adaptive swimming is not included in the modeling time), and begins formal continuous swimming for 21 days, bearing weight (4% of body weight) every day Exhausted swimming 1 time, 5-20 minutes a day, a total of 3 weeks of swimming.

[0109] Swimming conditions:

[0110] Swimming pool: diameter 200cm, hei...

Embodiment 3

[0131] Animal grouping: 36 healthy male SD rats with a body weight of (200 ± 20) g were purchased from Weitong Lihua Company, and were randomly divided into 3 groups: blank control group (8 rats), fed normally for 22 days; simple MCAO control group ( 12 rats), normal feeding for 3 weeks, suture embolization for 1h and reperfusion for 8h; composite model group (16 rats), control food intake, and oral propranolol (1mg / kg), continuous exhaustive swimming for 4w, then suture embolization 1 hour of ischemia and 8 hours of reperfusion.

[0132] Qi deficiency syndrome modeling method: the normal group does not swim; the composite model group swims adaptively after swimming for 2 days (adaptive swimming is not included in the modeling time), and begins formal continuous swimming for 21 days, bearing weight (6% of body weight) every day Swim 1 time exhaustively, 5-10 minutes a day, and swim for 2 weeks in total.

[0133] Swimming conditions:

[0134] Swimming pool: diameter 100cm, he...

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Abstract

The invention provides a method for manufacturing animal model of the qi-deficiency and blood-stasis disease, which particularly comprises the following steps: manufacturing an animal model of qi-deficiency syndrome by exhaustive exercise and/or basic food intake control and/or large dose of propranolol administration; and establishing a cerebral ischemia qi-deficiency and blood-stasis disease model by recovering blood supply of an artery after the qi-deficiency syndrome animal model blocks the artery in the brain.

Description

technical field [0001] The invention relates to a method for making an animal model, in particular to a method for making an animal model of Qi deficiency and blood stasis combined with disease and syndrome and an evaluation method thereof. Background technique [0002] Qi-deficiency and blood stasis syndrome is a common syndrome in many clinical diseases. During the years of objective and standardized research on Qi-deficiency and blood stasis syndrome, predecessors have initially revealed it from microscopic perspectives such as blood rheology, vascular regulatory substances, and histomorphological changes. The biological basis of qi deficiency and blood stasis syndrome. Qi is the most basic substance that constitutes the human body and maintains the life activities of the human body. Qi deficiency is a pathological change caused by insufficient generation and source of qi or excessive consumption of qi, which cannot function normally, resulting in decreased functional ac...

Claims

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Application Information

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IPC IPC(8): A01K67/027A01K67/02A61M31/00
Inventor 张允岭娄金丽扈新刚柳洪胜张锦李秀金香兰张志辰高铭坚宋莹
Owner DONGFANG HOSPITAL BEIJING UNIV OF CHINESE MEDICINE
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