Multi-scale integrated cell impedance sensor for detecting behavior of single cells and cell groups

Abstract

The invention discloses a multi-scale integrated cell impedance sensor for detecting the behavior of single cells and cell groups. Four interdigital electrode arrays are symmetrically distributed on an insulated substrate, and the edge parts of the insulated substrate are distributed with connection pads; a bus of each interdigital electrode array is respectively connected with the corresponding connection pad through a lead; microelectrodes are correspondingly distributed on at least an opening of the bus of each interdigital electrode array, and each microelectrode is connected with the corresponding connection pad through a lead; a culture container is provided with four culture cavities respectively matched with the areas where the four interdigital electrode arrays are positioned; a top cover is provided with four cavity covers respectively matched with the four culture cavities; the connection pads on the insulated substrate are connected with a PCB board; the four culture cavities of the culture container are respectively and correspondingly fixed on the areas where the four interdigital electrode arrays are positioned; and the four cavity covers are respectively and correspondingly arranged in the four culture cavities. The sensor not only improves the integration degree, but also can simultaneously detect the behavior of the single cells and the cell groups.

Description

technical field [0001] The present invention relates to the field of cell-based detection, and in particular to impedance sensors for detecting cell behavior. Background technique [0002] The rise of bioelectronics provides new and effective methods for the traditional detection of biological behavior of cells and biological macromolecules. Traditional biological detection methods include some biochemical and immunological methods, such as labeling with fluorescent dyes, which can detect a variety of characteristic proteins. MTT / XTT colorimetric method is used to determine the principle that tetrazolium salts (such as MTT, XTT, WST-1, etc.) can be reduced to purple crystalline substances by succinate dehydrogenase in the mitochondria of normal metabolic cells. reflect cell activity. The stronger the cell activity and proliferation ability, the more purple crystalline substances, and then read the OD value with a microplate reader to complete the test. However, such bioch...

AI Technical Summary

Problems solved by technology

For example, in the Chinese patent with the publication number CN1681938 published on October 12, 2005, and related reports contained in the patent, a variety of electrode arrays positioned on an insulating substrate are used to detect cells or molecules on the electrode surface. device that reflects the overall cellular response throughout the culture chamber but cannot precisely measure the behavior of individual cells

Images