Compound in salvia miltiorrhiza bungered sage root injection and application thereof in curing treating cardiovascular disease

A technology of Danshen injection and compounds, which is applied in the field of preparation of drugs for treating cardiovascular diseases and quality control of Danshen injection, and can solve problems such as not being able to meet the requirements of treatment standards for traditional Chinese medicine injections

Active Publication Date: 2010-06-02
CHIATAI QINGCHUNBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are already 66 manufacturers in China who have the drug approval number of Danshen injection, and the preparation methods refer to the ministerial standard WS 3 -B-3766-98-2004, its content and identification indicators are only protocatechualdehyde as an active ingredient, which obviously cannot meet the requirements of improving the treatment standards of traditional Chinese medicine injections

Method used

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  • Compound in salvia miltiorrhiza bungered sage root injection and application thereof in curing treating cardiovascular disease
  • Compound in salvia miltiorrhiza bungered sage root injection and application thereof in curing treating cardiovascular disease
  • Compound in salvia miltiorrhiza bungered sage root injection and application thereof in curing treating cardiovascular disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of embodiment 1. salvianolic acid M

[0030] Use Danshen medicinal material as raw material: take 1500g of Danshen, add 8 times the amount of water and decoct three times, the first time for 2 hours, the second and third times for 1.5 hours each, combine the decoctions, filter, and concentrate the filtrate to 750ml under reduced pressure. Add ethanol for precipitation twice, the first time to make the alcohol content 75%, and the second time to make the alcohol content 85%. 400ml, mix well, let stand, and filter. Using semi-preparative columns for Zorbax SB-C 18 (9.4mm×25cm, 5μm) was separated, purified, and freeze-dried to obtain a light yellow powder, namely 50.1 mg of the compound.

[0031] The mobile phase is composed of 0.05% formic acid-water (A) and acetonitrile (B), and the gradient elution program is: 0-28min, A-B (82:18, v / v); 28-60min, A-B (82:18-65 : 35, v / v), flow rate 4ml / min, for separation, retention time t R = 22.1 min.

Embodiment 2

[0032] The preparation method of embodiment 2. salvianolic acid M

[0033] Use Danshen medicinal material as raw material: take 1500g of Danshen, add 6 times the amount of water to decoct three times, 1.5 hours each time, combine the decoction, filter, and concentrate the filtrate to 750ml under reduced pressure. Add ethanol for precipitation twice, each to make the alcohol content 75%, filter after standing, recover ethanol from the filtrate, and concentrate to about 250ml extract. Dilute the extract with distilled water, let it stand, and filter. Use a semi-preparative chromatographic column to separate and purify, and freeze-dry to obtain a light yellow powder, that is, obtain 48.0 mg of the compound. The semi-preparative chromatographic column separation and purification are the same as in Example 1.

Embodiment 3

[0034] The preparation method of embodiment 3. salvianolic acid M

[0035] Use Danshen medicinal material as raw material: Take 1500g of Danshen, add 10 times the amount of water to decoct three times, 2.0 hours each time, combine the decoction, filter, and concentrate the filtrate to 750ml under reduced pressure. Add ethanol for precipitation twice, each to make the alcohol content 85%, filter after standing, recover ethanol from the filtrate, and concentrate to about 250ml extract. Dilute the extract with distilled water, let it stand, and filter. It was separated and purified by semi-preparative chromatographic column, and after freeze-drying, a light yellow powder was obtained, that is, 44.1 mg of the compound was obtained. The semi-preparative chromatographic column separation and purification are the same as in Example 1.

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Abstract

The invention discloses a new phenolic acid compound, i.e. danshinolic acid M extracted from salvia miltiorrhiza bungered sage roots, the compound also can be extracted from a salvia miltiorrhiza bungered sage root injection, and the invention simultaneously also discloses a method for extracting the new compound respectively from the salvia miltiorrhiza bungered sage roots and the salvia miltiorrhiza bungered sage root injection. Pharmacological studies indicate that the danshinolic acid M has myocardial preservation and oxidation resistant activity, thereby the compound has the prospect of being further developed into an effective medicament for curing treating cardiovascular diseases and can be used in the quality control standard of the salvia miltiorrhiza bunge red sage root injection.

Description

technical field [0001] The present invention relates to a new phenolic acid compound extracted from Danshen or Danshen injection, and also relates to the preparation method of this new compound and its use in the preparation of drugs for treating cardiovascular diseases and the quality control of Danshen injection . Background technique [0002] Danshen Injection is extracted and processed from the traditional Chinese medicine Salvia miltiorrhiza through modern technology. It is used for the prevention and treatment of cardiovascular and cerebrovascular diseases. The active ingredients are mainly phenolic acid compounds such as danshensu and salvianolic acid B, and there are still a large number of active ingredients that have not been discovered yet. [0003] There are already 66 manufacturers in China who have the drug approval number of Danshen injection, and the preparation methods refer to the ministerial standard WS 3 -B-3766-98-2004, its content and identification i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/86A61K31/343A61P9/00A61P39/06A61K9/08G01N30/02
Inventor 瞿海斌程翼宇范骁辉徐金钟莫必琪聂晶王治周天芳王木兰张晓丽
Owner CHIATAI QINGCHUNBAO PHARMA
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