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Method for detecting sarcosine

A detection method and sarcosine technology, which are applied in the detection field of chemical substances, can solve the problems of low separation efficiency of liquid chromatography and gas chromatography, high price of mass spectrometry detectors, limited use of chromatography-mass spectrometry, etc.

Active Publication Date: 2010-06-02
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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Problems solved by technology

In the prior art, the detection of sarcosine is mainly realized by chromatography-mass spectrometry, which combines the separation ability of chromatography with the qualitative function of mass spectrometry to achieve qualitative analysis of the mixture. Including liquid chromatography and gas chromatography, however, the separation efficiency of liquid chromatography and gas chromatography is low, and the mass spectrometry detector used in conjunction with them is expensive, which limits the use of chromatography-mass spectrometry
[0005] The capillary electrophoresis (CE) method has the advantages of simplicity, high efficiency, rapidity, and less sample consumption. The capillary electrophoresis electrochemical detection technology formed by applying electrochemiluminescence to capillary electrophoresis (CE) has become an ideal detection technology. This technology has successfully realized the analysis of substances such as proteins, amino acids, polypeptides, etc., but this method has not been applied in the prior art to realize the detection of sarcosine. Therefore, it is necessary to provide a capillary electrophoresis electrochemical method to realize Efficient, rapid and less expensive detection of sarcosine

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  • Method for detecting sarcosine
  • Method for detecting sarcosine
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Embodiment 1

[0044] In this embodiment, a sarcosine solution in which 10 μm sarcosine is dissolved in 1 mM acetic acid solvent is selected as the sample solution to be tested;

[0045] The electrophoresis buffer used in this embodiment includes a phosphate solution with a pH of 7-9, preferably a phosphate solution with a pH of 8.5 as the background buffer, and a phosphate solution with a pH of 9 as the running buffer.

[0046] The chemiluminescent reagent used in this embodiment is 5mM bipyridyl ruthenium (Ru(bpy) 3 2+ ).

[0047] All solutions were filtered through 0.22 μm polytetrafluoroethylene microporous membrane before use.

[0048] The concrete steps of this embodiment are as follows:

[0049] 1. Clean the electrophoresis capillary. The cleaning process can be washed with 1mol / L NaOH solution and 1mol / L HCl solution, then washed with deionized water, and finally washed with electrophoresis buffer to ensure that the inner wall of the electrophoresis capillary is clean. No other i...

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Abstract

The embodiment of the invention discloses a method for detecting sarcosine, which comprises the following steps: providing a capillary tube for capillary electrophoresis-electrochemiluminescence detection; adding a background buffer solution and pyridine ruthenium derivants into a capillary electrophoresis-electrochemiluminescence detection device; injecting a running buffer solution into the capillary tube, and under the condition that the running buffer solution is filled in the capillary tube, injecting a sample solution into the capillary tube; and applying voltages to the two ends of thecapillary tube to perform the capillary electrophoresis-electrochemiluminescence detection. In the method, the pyridine ruthenium derivants are selected as luminous reagents, and preferably, bipyridine ruthenium with high flexibility and high stability is utilized, so the highly-efficient and quick detection of the sarcosine is realized through simple apparatuses; and compared with gas chromatography-mass spectrometry, the method has the advantage of relatively low cost.

Description

technical field [0001] The invention relates to a method for detecting chemical substances, in particular to a method for detecting sarcosine. Background technique [0002] Sarcosine, also known as N-methylglycine or 2-methylaminoacetic acid, can be prepared by the reaction of chloroacetic acid and methylamine. Sarcosine usually exists in the muscle of the human body or animals in the form of phosphocreatine, which can improve the intelligence of the human body and increase the anaerobic and explosive power of the muscle. [0003] The article published in the "Nature" magazine (Nature 2009, 457: 910) on February 12, 2009 pointed out that sarcosine can effectively reflect the aggressiveness of cancer cells and can distinguish the growth behavior of cancer cells. After sarcosine was added to the culture medium of benign prostate cells, the benign prostate cells became aggressive and showed the biological characteristics of cancer cells, which indicated that sarcosine might be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 徐国宝李海娟
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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