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Biosensor for rapidly detecting sterigmatocystin and assembling method thereof

A biosensor, aspergillus versicolor technology, which is applied in the field of biosensors to achieve the effects of simple and convenient operation, sensitive response and strong anti-interference ability

Inactive Publication Date: 2010-06-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of biosensor still needs to be improved to improve its sensitivity, stability and reproducibility, etc.

Method used

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  • Biosensor for rapidly detecting sterigmatocystin and assembling method thereof
  • Biosensor for rapidly detecting sterigmatocystin and assembling method thereof
  • Biosensor for rapidly detecting sterigmatocystin and assembling method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Biosensor for Rapid Detection of Aspergillus versicolor

[0034] The biosensor for rapid detection of Aspergillus versicolor in actual samples according to the present invention includes a gold electrode (Au) as a base electrode and a reaction layer assembled on the gold electrode, and the reaction layer includes a polymer film, Electron transporter, chitosan gel and 6-methoxydifuranocoumarin oxidase. First, a layer of polymer film is assembled on the gold electrode by electrochemical method, and then the electron transporter is evenly dispersed in chitosan, and then 6-methoxybisfuranocoumarin oxidase is embedded in the electron transporter. In the mixture of body-chitosan, the formed electron transporter-6-methoxybisfuranocoumarin oxidase-chitosan hybrid was assembled on the gold electrode modified by the polymer film to form a 6- Methoxybisfuranocoumarin oxidase-modified biosensor.

[0035] In this example, poly-ortho-phenylenediamine was selected as the ...

Embodiment 2

[0036] Example 2: Preparation of a biosensor for detecting Aspergillus versicolor

[0037] (1) Preparation of materials

[0038] (1) Working electrode: a gold electrode with an inner diameter of 2mm was selected from Shanghai Chenhua Instrument Company.

[0039] (2) Ortho-phenylenediamine (OPD): chemically pure, purchased from Shanghai Chemical Reagent Co., Ltd.

[0040] (3) Single-walled carbon nanotubes (SWCNTs): diameter 2nm, length 5-15μm, purity 95%, purchased from Shenzhen Nanoport.

[0041] (4) Chitosan (CS): 85-90% degree of deacetylation, average molecular weight 1×10 6 g / mol, purchased from Zhejiang Aoxing Biotechnology Co., Ltd.

[0042] (2) Preparation of poly-o-phenylenediamine-modified gold electrodes

[0043] Put the gold electrode in 98% H by volume of 7 2 SO 4 and 3 volumes of 30% H 2 o 2 Soak in the formed solution for 30 minutes, rinse with double distilled water, and then polish to the mirror surface with alumina powder with a particle size of 1.0 μm,...

Embodiment 3

[0053] Example 3: Electrochemical response of MDCO / CS-SWCNTs / POPD / Au modified electrode to versicolor

[0054] The MDCO / CS-SWCNTs / POPD / Au modified electrode prepared in Example 2 was used as a working electrode, together with a common counter electrode and an optional reference electrode to form a three-electrode system.

[0055] In this example, an Ag / AgCl electrode (saturated KCl) was selected as the reference electrode, a Pt wire electrode was used as the counter electrode to form a three-electrode system, and 0.1mol / L PBS with a pH of 7.0 was used as the electrolytic support solution.

[0056] (1), cyclic voltammetry detection of ST

[0057] Using CHI660C electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd.), using a three-electrode system (reference electrode: Ag / AgCl electrode, counter electrode: Pt wire electrode, working electrode: MDCO / CS-SWCNTs / POPD / Au modified electrode), Select the cyclic voltammetry scan mode and set the electrochemical parameters a...

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Abstract

The invention discloses a biosensor for rapidly detecting sterigmatocystin and an assembling method thereof; the biosensor comprises a substrate electrode and a reaction layer assembled on the substrate electrode, the reaction layer comprises a polymer film, an electronic carrier, chitosan gel and 6-methoxy group furocoumarin coumarin oxidase, the 6-methoxy group furocoumarin coumarin oxidase is fixed in the electronic carrier-chitosan mixing membrane, and then is assembled on the substrate electrode decorated by the polymer film, thereby obtaining the biosensor for detecting the sterigmatocystin; the biosensor has high sensor sensitivity, rapid response time and strong anti-jamming capability, the detection lower limit to the sterigmatocystin can reach 3ng / ml, the biosensor can be used for practical sample detection, and the average recovery rate is 95.8 percent; the biosensor has simple manufacturing, convenient usage, good stability and long service life and provides a new detection method for rapidly detecting the sterigmatocystin content in practical samples.

Description

technical field [0001] The invention belongs to the field of biosensors, and relates to a biosensor for rapid detection of versicolor and an assembly method thereof. Background technique [0002] Sterigmatocystin (ST) is a carcinogenic and teratogenic mycotoxin, and is the synthetic precursor of aflatoxin (Aflatoxin, AFT). Connection composition. ST is a Class 2B carcinogen classified by the International Agency for Research on Cancer. It is closely related to lung cancer, liver cancer, and gastric cancer. It is the main source of fungal pollution in grains such as grain, forage grass, corn, wheat, and peanuts. In our country, most of the areas with high incidence of malignant tumors are due to the pollution of ST in food and grains. However, people have little understanding of the acute and chronic toxicity of ST, so the determination of the content of ST is of great significance. At present, the methods for determining ST mainly include thin layer chromatography (TLC), h...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 刘大岭陈俊华
Owner JINAN UNIVERSITY
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