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Hydrophilic fluorescent copper ion probe synthetic method

A technology of ion probe and synthesis method, which is applied in the field of synthesis of hydrophilic copper ion probe, can solve the problems of poor response and poor hydrophilicity, and achieve good effect, simple and feasible method, and simple pretreatment

Inactive Publication Date: 2011-10-26
SHANGHAI QIBAO HIGH SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantages of compounds a and b are that they have poor hydrophilicity, and they are not suitable for Cu in the aqueous solution system. 2+ poor response

Method used

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  • Hydrophilic fluorescent copper ion probe synthetic method
  • Hydrophilic fluorescent copper ion probe synthetic method
  • Hydrophilic fluorescent copper ion probe synthetic method

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0035] Experimental example 1.Selectivity analysis of Nth-Cu copper ion probe

[0036] Common heavy metal ions may interfere with the detection of copper ions, so it is necessary to determine whether the synthesized Nth-Cu copper ion probe can have good selectivity when recognizing copper ions. Add PtCl to the aqueous solution of 10mg / LNth-Cu copper ion probe 2 , AgNO 3 , Hg(NO 3 ) 2 、CuSO 4 , ZnSO 4 , Co(NO 3 ) 2 , MnSO 4 , NiC 2 o 4 , PbAc 2 Wait for the solid, and then use a fluorescence detector to measure the fluorescence emission intensity. Manually input the scanning wavelength range from 450 to 700nm, and the excitation wavelength is 365nm. For the relationship between the scanning wavelength and the fluorescence emission intensity, see Figure 5 , it can be seen that the Nth-Cu copper ion probe only responds to copper ions.

experiment example 2

[0037] Experimental example 2. Sensitivity analysis of Nth-Cu copper ion probe

[0038] Take 10mg / L Nth-Cu copper ion probe solution as the standard storage solution, pipette 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, 10.0, 20.0ml solutions respectively, dilute to 100ml, and obtain 0.01, 0.01, 0.05, 0.1 , 0.2, 0.5, 1.0, 2.0mg / L probe solution. Sampling, under the excitation of ultraviolet light (wavelength 365nm) respectively, visual observation phenomenon. It is found that the naked eye can perceive the copper ion probe solution to show obvious fluorescence concentration is above 0.5mg / L, if the concentration is lower than 0.5mg / L, the visual fluorescence effect is not good. Then 1 mg / L copper sulfate solution was added dropwise in the 1 mg / L copper ion probe solution. It can be seen that the visual sensitivity of the Nth-Cu copper ion probe solution is very good. If a fluorescence detector is used, a lower concentration of copper ions can be detected, the lowest can reach 1×10-7mol / L....

experiment example 3

[0039] Experimental example 3. Quantitative relationship analysis between Nth-Cu copper ion probe and copper ion when fluorescence is quenched

[0040] When equal volumes of Cu2+ solutions with different concentrations are added, from the change of the fluorescence of the Nth-Cu copper ion probe, it is found that when the concentration of copper ions is small, the fluorescence of the probe is not completely quenched; when the concentration of the copper ion probe and the copper ion substance When the concentration of copper ions is higher or the concentration is higher, the fluorescence is completely quenched. That is, the Nth-Cu copper ion probe is combined with copper ions in a 1:1 weak bond, and the range of copper ion concentration can be detected by using this characteristic. Nth-Cu copper ion probe interacts with copper ions, such as Figure 6 , the introduction of copper ions in the frame forms a weak bond to block intramolecular electron transfer, resulting in fluoresce...

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Abstract

The present invention relates to a hydrophilic fluorescent copper ion probe and a synthetic method thereof. The synthetic steps comprises: brominating, nitrifying, oxidizing sundex side chain, inserting hydrophilic group, removing nitro and forming cavity. The hydrophilic fluorescent copper ion probe obtained by the synthetic method has hydrophilic characteristics and good selectivity and sensitivity, pre-treatment is not necessary for the detection of the samplings or very simple; and the method for detecting copper ions quantitatively is simple and feasible, the fluorescent change effect by visual inspection is good with wide application prospect.

Description

technical field [0001] The invention relates to a copper ion detection reagent used in measuring copper ions by a fluorescence method and a synthesis method thereof, in particular to a synthesis method of a hydrophilic copper ion probe. Background technique [0002] Copper is one of the essential trace elements for the human body, animals and plants, and plays an important role in regulating the metabolism of the body. However, excessive intake of copper can cause copper retention in the tissue and accumulate in the liver and brain, which can cause tissue lesions. At present, the commonly used copper ion detection methods are copper reagent (DDTC) method, spectrophotometry, atomic absorption method and so on. These methods are more sensitive, but there are disadvantages such as time-consuming, testing samples need to be pre-treated, samples are easily lost, and the results are low. [0003] Fluorescence is one of the practical methods to eliminate the shortcomings of commo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76C09K11/00
Inventor 黎良枝吴纪舫高俊
Owner SHANGHAI QIBAO HIGH SCHOOL