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Construction of Brassica napus recessive nuclear sterile near-isogenic line and single cross hybrid production technology

A technique for recessive genital sterility and Brassica napus, applied in plant genetic improvement, botanical equipment and methods, applications, etc., can solve problems such as unfavorable rape mechanization, light simplification, increased harvest loss of rapeseed, and reduced heterosis , to achieve strong heterosis, simplified cross-breeding procedures, consistent flowering and maturity

Inactive Publication Date: 2012-01-04
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing recessive genital sterile "three-line method" seed production technology, due to the differences in the genetic backgrounds of the homozygous two-type line and the temporary insurance line, the total sterile line produced is actually a hybrid, and then the all-sterile line is actually a hybrid. The commercial hybrids produced by crossing the sterile line with the restorer line with different genetic backgrounds are actually three-crosses. Due to the serious separation of the genetic background of the three-crosses, the heterosis of recessive sterility in Brassica napus is reduced. There are differences in the flowering and maturity stages of different individual plants in production, and the consistency is poor, which will increase the loss of rapeseed harvest, and is also not conducive to the mechanization and simplification of rapeseed.

Method used

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  • Construction of Brassica napus recessive nuclear sterile near-isogenic line and single cross hybrid production technology
  • Construction of Brassica napus recessive nuclear sterile near-isogenic line and single cross hybrid production technology
  • Construction of Brassica napus recessive nuclear sterile near-isogenic line and single cross hybrid production technology

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Experimental program
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Effect test

Embodiment 1

[0042] Discovery of "Shanghai Youza No. 5"

[0043] The temporary insurance line M-6029, whose genotype is aabbrfrf, is the temporary insurance line of recessive genetic male sterile 20118A, which was passed by Sun Chaocai of Shanghai Academy of Agricultural Sciences in 1999 in the test cross combination of recessive genetic male sterile 20118A. Cross, bred by test cross, with erucic acid content of 0.89%, glucosinolate content of 25.36μmol / g, excellent agronomic characters, high general combining ability and special combining ability.

[0044]In order to use M-6029 to select a strong dominant hybrid combination, in 2000, the M-6029×99105 (quoted from Jiangsu Academy of Agricultural Sciences) hybrid combination was manually configured by manual emasculation, and the combination was found to perform well through small-area comparative experiments .

Embodiment 2

[0046] Breeding of "Huyouza No. 5"

[0047] 1. Improve the two-type line 20118AB by backcrossing the temporary insurance line M-6029

[0048] At the flowering stage of rapeseed in March 2001, the fertile line 20118B of the two-type recessive male sterile line was used as the female parent to cross with the Lingbao line M-6029, and the offspring could be separated 1 fertile plant: 1 sterile plant; 20118B The sterile line in the ×M-6029 hybrid progeny was backcrossed with the recurrent parent M-6029, and the offspring were always separated into 1 fertile plant (aabbrfrf) : 1 sterile plant (aabbRfrf). After 5 consecutive generations of backcrossing, the fertile The genetic background of strains and sterile strains tended to be consistent with that of the temporary insurance line M-6029 (see image 3 ).

[0049] The fertile line in the offspring of 20118B×M-6029 was backcrossed with the recurrent parent M-6029, and the offspring were always segregated as 1 fertile plant (AabbRfr...

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Abstract

The invention discloses construction of Brassica napus recessive nuclear sterile near-isogenic line and a single cross hybrid production technology. A fertile plant of a recessive sterile homozygous two-type line serves as a donor, and a temporary maintainer line serves as a recurrent parent for repeated rotation cross to obtain a new homozygous two-type line having the same genetic background with the temporary maintainer line; the sterile plant of the new recessive nuclear sterile homozygous two-type line and the temporary maintainer line with the same genetic background are hybridized to produce a complete sterile line; and then the complete sterile line and a restoring line are hybridized to produce a single cross hybrid. In the invention, the genetic background of the homozygous two-type line and the temporary maintainer line are quickly homogenized so that a commercial F1 hybrid is converted into the single cross hybrid from the original three way cross hybrid and is applied to production.

Description

technical field [0001] The invention relates to the field of Brassica napus breeding, in particular to the construction of a recessive genic male sterile near-isogenic line of Brassica napus and the single-cross production technology. Background technique [0002] 1) Inheritance of recessive nuclear sterility in Brassica napus [0003] The recessive sterility of Brassica napus is controlled by 3 pairs of recessive genes. In order to describe its inheritance, we named the three recessive genes as a, b and rf respectively, where a and b are recessive sterility genes, rf is a recessive epistatic gene, which can inhibit the expression of a and b sterility genes and restore fertility to fertility. The sterile plants had two genotypes, aabbRfRf was homozygous sterile plants, and aabbRfrf was heterozygous sterile plants. There are 25 genotypes of fertile plants, among which the segregation ratio of aaBbRfRf and AabbRfRf is 3:1, the ratio of aaBbRfrf and AabbRfrf is 13:3, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 孙超才杨立勇王伟荣周熙荣李延莉蒋美艳庄静顾龙弟
Owner SHANGHAI ACAD OF AGRI SCI
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