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Cereals alcohol-soluble protein conduit and preparation method thereof

A gliadin and protein tube technology, used in catheters, medical science, prostheses, etc., can solve the problem of difficulty in meeting the flexibility of nerve catheters or blood vessels, poor mechanical properties of chitosan and collagen, and no preparation of zein. Protein hollow tube and other problems, to achieve the effect of good biodegradability, uniform roughness, and easy to achieve

Active Publication Date: 2013-02-27
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the inherent defects of the above-mentioned conventional biomaterials: poor mechanical properties of chitosan and collagen, it is difficult to meet the flexibility required by nerve conduits or blood vessels; inflammatory reactions caused by local acidity after degradation of polylactic acid; conduits do not have electrical conductivity. sex etc.
At the same time, there are also the following defects in the process of preparing the catheter: special molds are required; toxic cross-linking agents are used in the process of forming the tube; drying under special conditions that consume energy is required.
The application of electrospinning technology has broadened the processability of zein, however, there is no report on the preparation of zein hollow tubes and their application in the field of tissue engineering tubes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A 0.28mm diameter polytetrafluoroethylene rod was used as the extraction mold, and the protein solution (100mg / ml~150mg / ml, ethanol content 60%~65%) was extracted for 4~7 times, and the mold was removed after it was completely dried ( It can be seen that the whole body of the protein tube is transparent); extract the grain gliadin microsphere solution again (grain gliadin 20mg / ml, ethanol content 40%); after it is completely dry, immerse it in a 1mol / L citric acid aqueous solution, and incubate at room temperature for 1 After ~2 hours of drying, a cereal-like prolamin tube that can meet the size and mechanical requirements of bridging rat optic nerves is obtained. The inner diameter of the protein tube is 0.28mm, the average wall thickness is 27μm, the tube length is ≤1cm, the shape of the tube body is good, and the microspheres on the tube wall are evenly distributed. The cereal prolamin is a prolamin derived from corn.

Embodiment 2

[0035] A 1.6mm diameter polytetrafluoroethylene tube was used as the extraction mold, and the protein solution (150mg / ml~200mg / ml, ethanol content of 65%~70%) was extracted 7~10 times, and the mold was removed after it was completely dried ( It can be seen that the whole body of the protein tube is transparent and uniform); extract the grain gliadin microsphere solution again (grain gliadin 20mg / ml, ethanol content 40%); after it is completely dry, immerse it in 1mol / L citric acid aqueous solution, room temperature After incubating for 2-3 hours and drying, a cereal-like prolamin tube that can meet the size and mechanical requirements of bridging the rat sciatic nerve is obtained. The inner diameter of the protein tube is 1.6mm, the average wall thickness is 129μm, the tube length is ≤3cm, the tube body shape is good, and the tube wall microspheres are dense and uniform. The cereal prolamin is a prolamin derived from corn.

Embodiment 3

[0037] A 12mm diameter polytetrafluoroethylene tube was used as the extraction mold, and the protein solution (250mg / ml~300mg / ml, ethanol content 70%~80%) was extracted 7~10 times, and the mold was removed after it was completely dried (visible The whole body of the protein tube is transparent); extract the grain prolamin microsphere solution again (grain gliadin 20mg / ml, ethanol content 40%); after it is completely dry, immerse it in a 1mol / L citric acid aqueous solution, and incubate at room temperature for 3~ After 4 hours of drying, polyaniline was deposited on the tube body again, and the deposition time was 12 hours, so that the grain-like prolamin tube with electrical conductivity was obtained. The inner diameter of the protein tube is 12mm, the average wall thickness is 85μm, the tube length is ≤5cm, the tube shape is good, the whole body is green and opaque, and the resistance value is 3.9×103Ω, which can meet the electrical conductivity requirements of nerve tissue in...

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Abstract

The invention relates to a cereals alcohol-soluble protein conduit and a preparation method thereof in the field of biomedical technology; the method comprises the following steps: cereals alcohol-soluble protein and organic solvents are mixed to obtain protein solution; after the protein solution is extracted by a mold, drying treatment is carried out and the protein pipe is demoulded from the mold; secondary extraction is carried out to the protein pipe, so as to form a nanoscale spherical structure surface outside, and then form fixation is carried out; finally, conducting polymer is deposited on the protein conduit surface, so as to prepare the conductive cereals alcohol-soluble protein conduit. In the invention, the cereals alcohol-soluble protein is used as raw materials, and the protein conduit has good biocompatibility and degradation, is suitable for preparing tissue engineering conduits and is helpful for regeneration and functional rehabilitation of damaged tubular shaped soft tissues.

Description

technical field [0001] The invention relates to a material in the technical field of biomedicine and a preparation method thereof, in particular to a cereal-like prolamin tube with an inner diameter of 0.2-12 mm and a tube length of ≤5 cm and a preparation method thereof. Background technique [0002] The emergence of the concept of tissue engineering and its development in the past two decades has given us hope for the complete treatment of damage and dysfunction of human tissues and organs, a new technique for directly obtaining new tissues and new organs through cell culture, which is considered to be 21 The most promising therapeutic method in the century [Liu Q, Tissue Engineering (tissue engineering), In: Biomaterials and Tissue Engineering, Shi DL ed, Beijing, Tsinghua University Publishing Press, 2004, p.195.]. In the field of tissue engineering research, biocompatible and degradable materials are usually used to induce regeneration of surrounding tissue or become a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L29/12A61L27/48
Inventor 王瑾晔狄伶
Owner SHANGHAI JIAOTONG UNIV