Novel insect pest resistant bacillus thuringiensis strain
A kind of Bacillus aureus, pest technology, applied in the field of novel Bacillus thuringiensis strains
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Embodiment 1
[0067] Embodiment 1: Beet armyworm (Spodoptera exigua)
[0068] After a large amount of Bacillus thuringiensis A603 was fermented, 5 liters of the fermentation culture was taken for serial dilution, and the fermentation culture of different dilution concentrations was tested by the feed mixing method to test the biological activity of the third instar larvae of beet armyworm for 120 hours. (the number of tested worms in each group of experiments is 20, and there is a continuous observation of changing feed). After recording the number of dead worms before and after being treated with Bacillus thuringiensis, the larval mortality rate is calculated.
[0069] Please refer to Table 1, which is the mortality test data of Bacillus thuringiensis A603 against beet armyworm. From the results, it can be known that Bacillus thuringiensis A603 does have a pest-killing effect.
[0070] Table 1. Activity data of Bacillus thuringiensis A603 fermentation culture against beet armyworm
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Embodiment 2
[0073] Embodiment two: Spodoptera litura (Spodoptera litura)
[0074] The test treatment method is the same as that in Example 1. The fermented culture after the diluted concentration was tested with the feed mixing method to process the third instar early larvae of Spodoptera litura for 120 hours in a row (the number of test worms in each group of experiments was 20, and there was continuous observation of changing feed), and the records were recorded with Bacillus thuringiensis. The weight of worms and the number of dead worms before and after Bacillus treatment.
[0075] Please refer to Table 2, which is the activity inhibition data of Bacillus thuringiensis A603 against Spodoptera litura. The average insect weight of Spodoptera litura before treatment was 0.0025 g. The growth multiple is the average insect weight after treatment divided by the average insect weight before treatment. Inhibition rate (%) is the difference between the average insect weight of the control g...
Embodiment 3
[0081] Example three: Bean borer (Maruca vitrata)
[0082] The test treatment method is the same as that in Example 1. Fermentation cultures with different dilution concentrations were tested and treated with the feed mixing method to treat the second instar and the third instar early larvae of the bean moth for 120 hours in a row (five larvae for each concentration, continuous observation without changing the feed), and recorded the 24th to 3rd instar larvae. The number of dead bugs in 120 hours.
[0083] From the distribution results of the lethality rate in Table 3, it can be known that Bacillus thuringiensis A603 can achieve nearly 50% resistance to bean borer larvae even at low treatment concentrations after 96 hours of treatment with larvae.
[0084] Table 3. Activity data of Bacillus thuringiensis A603 fermentation culture against bean borer
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