Microfluidic sample feeding method, device and application thereof

Abstract

The invention discloses a microfluidic sample feeding method, a device and an application thereof. The device mainly comprises amicrofluidic chip with the structure being of a cross-shaped microchannel, a liquid storage tank, a connection pipe, a tee electromagnetic valve, a pressure generating unit, a signal detection unit and a host machine. The device is characterized in that gate control state: negative pressure is applied to the bw end and the sw end to drive the microfluid so as to form a hydraulic gate control at the center of the cross-shaped channel, i.e. all samples at an s end flowto the sw end, and the buffering liquid on the b end flows into the bw end and the sw end. The distribution ratio of the buffering liquid at the bw end and the sw end is controlled by the pressure ratio on the pressurizing end. The samples on the s end flows into the channel o-bw by instantly increasing the pressure ratio of the bw end and the sw end. The sample feeding volume can be controlled by adjusting the pressure ratio and the feeding time. The instrument automatically returns to the hydraulic gate control state after the sample feeding at each time, and the fed sample can be separated, detected and analyzed on the o-bw end. By utilizing the method and the instrument, the medicine irritate test of the cell can be performed.

Description

technical field [0001] The invention relates to a microfluidic sample injection method, device and application thereof. The method is a liquid sample injection method for microfluidic chip epithelial upgrade to nanoliter upgrade, and can be applied to sample separation on microfluidic chip , cell stimulation and other biochemical analysis. Background technique [0002] In recent years, micro-total analysis system (micro-total analysis system, μ-TAS) or lab on a chip (Lab on a chip) has had a significant impact in the fields of chemistry, physics, biology and bioengineering. Based on microfluidic and nanofluidic control technologies, the micro-total analysis system guides the development of analytical instruments in the direction of miniaturization, integration, automation, and high-throughput rapid quantitative analysis. The micro-total analysis system provides a possible strategy for real-time detection and on-site analysis of biochemical environmental samples, and it also...

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of sample injection in the existing micro-total analysis system (such as the discriminatory sampling method, damage to living biological samples, and non-adjustable sample volume), and provide a micro-liter to nanoliter Microfluidic sample injection method, device, and application of the method in liquid chromatography separation and cell stimulation experiments

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