Enzyme sensing determination method for indoleacetic acid content in plant sample

An enzyme sensor and sample technology, which is applied in the direction of measuring devices, electrochemical variables of materials, instruments, etc., can solve the problem that the reproducibility of ELISA is greatly affected by environmental conditions, low specificity and sensitivity, long measurement cycle, etc. problem, to achieve the effect of improving complexity and sensitivity, improving accuracy and sensitivity, and fast and highly sensitive detection

Inactive Publication Date: 2010-07-28
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

Among them, the biological test method is simple and easy, and can reflect the physiological activity of the tested plant hormones, but the influence of plant hormone analogs and antagonists cannot be ruled out, and the specificity and sensitivity are low; the chromatography method has high sensitivity and good reproducibility , but requires a large amount of samples to be tested, complex sample pretreatment, expensive equipment and high mainte

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  • Enzyme sensing determination method for indoleacetic acid content in plant sample
  • Enzyme sensing determination method for indoleacetic acid content in plant sample

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[0013] The present invention will be further described below in conjunction with example:

[0014] 1. Reagents and instruments:

[0015] IAA, purchased from Sigma-Aldrich Company. Sodium dihydrogen phosphate, disodium hydrogen phosphate, quartz sand, absolute ethanol, thionine, sodium acetate, glacial acetic acid, and 25% glutaraldehyde were purchased from Shanghai Chemical Reagent Company.

[0016] The current detection process was carried out on a CHI760c electrochemical workstation (Shanghai Chenhua Instrument Company). In the three-electrode system, a platinum electrode (CHI102, 2 mm in diameter, Shanghai Chenhua Instrument Co., Ltd.) was used as the working electrode, a platinum wire electrode (CHI115, Shanghai Chenhua Instrument Co., Chenhua Instrument Company) as the reference electrode. BRANSONIC 200 Ultrasonic Cleaner (BRANSON ULTRASCHALL, Germany), CS 501-SP Super Digital Display Thermostat (manufactured by Chongqing Sida Experimental Instrument Factory), HP300GS-...

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Abstract

The invention relates to an enzyme sensing determination method for indoleacetic acid content in plant sample, which includes the following steps: (1) enzyme sensor building: a platinum electrode is sequentially rubbed and polished by metallographic abrasive paper and muntjac skin with alumina suspension solution to form mirror surface, and the cleaned platinum electrode is performed with thionine electropolymerization and glutaraldehyde covalent cross-linking oxidase; (2) standard curve drawing: a series of IAA with different concentrations can be determined by the enzyme sensor, and the standard curve is drawn according to the relationship of current change and IAA concentrations; (3) IAA content detection of sample: the enzyme sensor is adopted to detect the current change of the sample, and the IAA concentration is calculated according to the standard curve. The invention has the beneficial effects that: the enzyme sensing determination can quickly and accurately determine the IAA content of the plant sample, compared with the traditional detection method, the enzyme sensing determination method is simple, obtains reliable results and has low determination cost.

Description

technical field [0001] The invention belongs to the field of plant hormone detection, and in particular relates to an enzyme sensing method for measuring the content of indole acetic acid in plant samples. Background technique [0002] Since the discovery of auxin in 1928, the research related to plant hormones has involved almost all fields of botany research. The study of plant hormones and their mechanism of action has become an important way for people to understand and understand plant life phenomena, providing a basis for crop genetic improvement. It provides a strong theoretical support and has played an irreplaceable role in promoting the "green revolution", greatly increasing crop yields and ensuring national food security. In recent years, with the development of plant molecular biology and genomics, breakthroughs have been made in the genome research of model plants represented by Arabidopsis thaliana and rice, and the research on plant hormones, which is at the f...

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 肖浪涛黄志刚王若仲丁君辉刘清
Owner HUNAN AGRICULTURAL UNIV
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