Pesticide biological activity determination method

Abstract

The invention discloses a pesticide biological activity determination method which comprises the following steps that: (1) a sample (pesticide raw medicine or plant extract) is prepared into 10g/L mother solution by distilled water, the sample which is insoluble in water is first added with a small amount of acetone to be dissolved, and is gradually diluted by artificial seawater to the sample concentration; (2) 10mu L of artemia liquid is shifted to a 24-hole culture plate, and then 1.5ml sample liquid pesticide to be tested is added in to be put into a 25DEG C light incubator to be cultured, and no bait needs to be fed during the test; each treatment is repeated for four times, and simultaneously, the artificial seawater which contains a certain amount of acetone is set for reference; and (3) after 48h of culture, the number of dead artemia nauplii is observed under a anatomical lens, the corrected mortality is calculated, and the insecticidal biological activity of a sample to be tested is evaluated. The method provided by the invention has the advantages of sensitivity to the activity of most pesticide, wide sources, easy largely feeding, low determination cost, simple and convenient method and trace amount of required sample, and is particularly applicable to the screening or the determination of the trace amount of sample.

Description

Technical field: [0001] The invention belongs to the field of pesticide bioassay, in particular to a bioassay method for pesticide insecticidal activity using Artemia Salina as a model organism. Background technique: [0002] Biological activity screening is an important part of pesticide research and development, which is directly related to the efficiency and success of new pesticide development. The traditional bioassays of insecticidal activity mainly use standard test insects such as armyworm, housefly, trichopinum, rice weevil, aphid, corn borer, diamondback moth, and cabbage butterfly larvae as target organisms. These model organisms have high requirements on environmental conditions, and the cultivation and mass supply of test insects is a very professional and difficult task. In addition, the above-mentioned test organisms are relatively large, consume a large amount of test materials and compound samples, occupy a large experimental space, and are labor-intensive....

AI Technical Summary

Problems solved by technology

However, the use of Artemia in the bioassay of pesticide insecticidal activity is rarely used, a

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