Colorimetric detection method and device of sugar content of water

Abstract

The invention relates to a colorimetric detection method of sugar content of water, which comprises the steps of: under the condition of concentrated sulfuric acid, taking alpha-naphthol as a color-developing agent to prepare furfuraldehyde and derivatives thereof under the action of sugar and the concentrated sulfuric acid, and further performing condensation of the furfuraldehyde and the derivatives thereof and the alpha-naphthol to obtain purple complex compounds; and measuring reactant and recording change of intensity of transmitted light of aubergine condensation compounds absorbing absorb light with wavelength of 595 nanometers by a photoelectric colorimeter to obtain a response curve with corresponding peak height and peak width, and comparing the peak height of the curve with that of a light absorption curve of master standard water with a known sugar concentration to obtain sugar content values in water samples through calculation. The method has the advantages that: the on-line detection of the content of sugar with low content in water or solution can be performed; the process is simple, convenient and short; the detection data is accurate and reliable; the sample injected for one time of detection is only 50mu m to 1ml; only a very small amount of reagent is consumed each time; the operation cost is low; and the reagent is safe and nontoxic.

Description

technical field [0001] The invention relates to a colorimetric detection method for sugar content in water, and in particular to a sugar colorimetric detection method capable of measuring trace amounts of sugar in an aqueous solution or monitoring on-line, belonging to the fields of chemical analysis and water environment monitoring and analysis. Background technique [0002] Sugars are generally divided into monosaccharides, disaccharides and polysaccharides. When sugar dissolves in water, monosaccharides and disaccharides containing hemiacetal hydroxyl groups can undergo tautomerism in aqueous solution, and the open-chain structure and ring structure have a certain balance, so they are reductive, also known as reduction sugar. Polysaccharides such as sucrose, starch, and cellulose are non-reducing sugars, but they can be hydrolyzed into monosaccharides under the action of acid or enzymes, so their hydrolyzate has reducing properties. [0003] Excessive sugar content in w...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for detecting sugar in water with simple operation, fast and reliable, non-toxic reagents and low operating costs and a low-content sugar detection device specially used for the method, so as to overcome the traditional anthracene-copper colorimetric method. Low-content sugar detection methods such as Lin's volumetric method have disadvantages such as cumbersome operation, toxic reagents, or reagents that cannot be stored for a long time, and realize automatic online detection of sugar in water

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