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Method for detecting flavone in pectin

A determination method and technology of flavonoids, which are applied in the preparation of test samples, measurement of color/spectral properties, and material analysis by observing the influence on chemical indicators, can solve the problems of inaccurate detection results of flavonoids in pectin. , to achieve the effect of good extraction effect, accurate and reliable data results, and outstanding substantive characteristics

Inactive Publication Date: 2010-09-01
YANTAI ANDRE PECTIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the above-mentioned deficiencies in the prior art, and provide a kind of assay method of flavonoids in pectin, mainly solve the problem of inaccurate detection result of flavonoids in existing pectin

Method used

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  • Method for detecting flavone in pectin
  • Method for detecting flavone in pectin
  • Method for detecting flavone in pectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1, weigh 11.3766g of the sample, add 20mL of 0.1mol / L NaOH to fully stir and dissolve, adjust to pH=12 with 4mol / L NaOH, adjust to pH=6 with 20% citric acid, add water to volume to 100mL, filter with filter paper; collect the clarified sample solution for later use;

[0017] Accurately draw 2 mL of 2 samples into 2 volumetric flasks, add 3 mL of blank reagent, add 5 mL of 90% diethylene glycol and 0.1 mL of 4mol / L NaOH, and add 0.1 mL of 4 mol / L NaOH to the sample blank, shake well, and place Incubate and develop color in a water bath at 40°C for 10 minutes, then immediately pour it into a 1cm cuvette and measure its extinction value at a wavelength of 420nm; subtract the extinction value without alkali from the extinction value with alkali, and check the standard curve to obtain the sampled solution The number of milligrams of flavonoids in;

[0018] Prepare the standard solution as needed, and draw the standard curve; and calculate the result, and bring the o...

Embodiment 2

[0024] Example 2, according to the flavonoid content in pectin, weigh 0.1g (W) of the solid sample, add 20mL of 0.1mol / L NaOH to fully dissolve it, adjust the pH to 12 with 4mol / L NaOH, and use 20% lemon Adjust the acid to pH=6, add water to make up to 100mL, and filter through filter paper; collect the clarified sample solution for later use;

[0025] Accurately draw 2 mL of 2 samples into 2 volumetric flasks, add 3 mL of blank reagent, add 5 mL of 90% diethylene glycol and 0.1 mL of 4mol / L NaOH, and add 0.1 mL of 4 mol / L NaOH to the sample blank, shake well, and place Incubate and develop color in a water bath at 40°C for 10 minutes, then immediately pour it into a 1cm cuvette and measure its extinction value at a wavelength of 420nm; subtract the extinction value without alkali from the extinction value with alkali, and check the standard curve to obtain the sampled solution The number of milligrams of flavonoids in;

[0026] Prepare the standard solution as needed, and dr...

Embodiment 3

[0032] Example 3, according to the content of flavonoids in pectin, weigh 20 g (W) of flavonoid liquid samples, add 20 mL of 0.1 mol / L NaOH to make it fully dissolved, adjust the pH to 12 with 4 mol / L NaOH, and use 20% lemon Adjust the acid to pH=6, add water to make up to 100mL, and filter through filter paper; collect the clarified sample solution for later use;

[0033] Accurately draw 2 mL of 2 samples into 2 volumetric flasks, add 3 mL of blank reagent, add 5 mL of 90% diethylene glycol and 0.1 mL of 4mol / L NaOH each, and add 0.1 mL of 4 mol / L NaOH to the sample blank, shake well, and place Incubate and develop color in a water bath at 40°C for 10 minutes, then immediately pour it into a 1cm cuvette and measure its extinction value at a wavelength of 420nm; subtract the extinction value without alkali from the extinction value with alkali, and check the standard curve to obtain the sampled solution The number of milligrams of flavonoids in;

[0034] Prepare the standard ...

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Abstract

The invention discloses a method for detecting flavone in pectin. The method is characterized by comprising the following steps of: a, pectin sample pretreatment; b, absorbance detection; c, standard curve drawing; and d, result calculation. The method has simple operation and accurate detection result, and can accurately and effectively detect the flavone in the pectin.

Description

Technical field: [0001] The invention relates to the technical field of pectin detection, in particular to a method for determining flavonoids in pectin. technical background: [0002] At present, the extraction liquid used in the detection of flavonoids is the organic solvent benzene and methanol or water. Both methods have defects. The organic solvent and methanol method are used to extract not only flavonoids, but also impurities. , so that there are many interfering substances, and the test results are inaccurate; and when water is used as the extraction solution, the flavonoids cannot be fully extracted, and the test data is not accurate. Invention content: [0003] The purpose of the present invention is to overcome above-mentioned deficiencies in prior art, and provide a kind of determination method of flavonoids in pectin, mainly solve the problem that the detection result of flavonoids in existing pectin is inaccurate. [0004] In order to achieve the above objec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N1/28
Inventor 张安明胡晓燕李霖善房德刘冰杜启印杨秀军
Owner YANTAI ANDRE PECTIN
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