Selective medium of liver stem cells, method for selectively separating and amplifying liver stem cells as well as medicinal composition for treating diabetes

A technology for selecting medium and hepatic stem cells, which is applied in the fields of selective medium for hepatic stem cells, selective separation and expansion of hepatic stem cells and pharmaceutical compositions for treating diabetes, and can solve the problem of poor safety of hepatic stem cells and low purity of hepatic stem cells , easy differentiation and degeneration, etc., to overcome diabetes-dependent insulin therapy, strong selectivity, high-efficiency separation and large-scale amplification
CN101851605AInactive Publication Date: 2010-10-06北京弘润天源基因生物技术有限公司
4 Cites 13 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京弘润天源基因生物技术有限公司
Publication Date
2010-10-06
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a selective medium of liver stem cells. The selective medium comprises a liquid-based medium, and the volume of the liquid-based medium is used as a standard. The selective medium also comprises 1-20vol% of fetal calf serum, 5-100ng / mL of human epidermal growth factors and 5-100ng / mL of basic fibroblast growth factors. The invention also provides a method for selectively separating and amplifying liver stem cells from in vitro tissues by using the selective medium. The invention also provides a medicinal composition for treating diabetes, which contains the liver stem cells prepared by using the method. The method using the selective medium can separate and amplify liver stem cells in high efficiency and in a large amount, and the liver stem cells subcultured and amplified through 10 generations still maintain the same properties with the primary liver stem cells. The medicinal composition for treating diabetes can restore damaged islet beta cells of a person with diabetes, and can eliminate insulin resistance.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a selection medium for stem cells, a method for selectively isolating and expanding stem cells using the medium, and a pharmaceutical composition for treating diabetes comprising the stem cells obtained by the aforementioned method. More specifically, the present invention relates to a selective medium for hepatic stem cells, a method for selectively isolating and expanding hepatic stem cells using the medium, and a pharmaceutical composition for treating diabetes comprising the hepatic stem cells obtained by the aforementioned method. Background technique

[0002] The existing methods for isolating and expanding hepatic stem cells are complex and costly, and the isolated hepatic stem cells have low purity, low expansion efficiency, and easy differentiation and denaturation, and the culture medium used in the isolation and expansion process contains many Harmful drugs make the isolated and expanded hepatic stem cells le...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More