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Production process for separating and extracting subprostrate sophora polysaccharide by enzymatic hydrolysis method

A production process and technology of enzymatic hydrolysis, which is applied in the field of separation and extraction of soybean root polysaccharides by enzymatic hydrolysis, can solve the problems of low production efficiency, insufficient supply, and low total sugar content, and achieve the effect of improving efficiency and meeting market demand

Inactive Publication Date: 2012-10-03
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current water-extraction and alcohol-precipitation method for the separation and extraction of polysaccharides from bean root has the problems of low polysaccharide yield and low total sugar content, so that the production efficiency is low, the supply is insufficient, and it is difficult to meet the market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] A) Accurately weigh 4 g of crushed pea root, add 60 mL of distilled water, and soak for 0.5 h; B) adjust the pH of the soaking solution in step A) to 5.0, add papain to make the mass volume concentration 4%, and mix well , placed in a water bath at 60°C for 150 minutes, filtered, and the filtrate was used for later use; the residue was added to 60 mL of distilled water, placed in a water bath in boiling water, filtered, and the filtrate was used for later use; 95% ethanol twice as much as the concentrated solution to make the alcohol concentration reach 80%. After mixing well, let stand overnight at low temperature for more than 12 hours, pour out the supernatant, and centrifuge at 3000r / min for 10min to obtain the precipitate; D) take the precipitate in step C) and press Add distilled water to dissolve at a mass volume ratio of 1:5, let stand, centrifuge at 3000r / min for 10min, pour out the supernatant for later use; E) take the supernatant in step D), add 5 times the a...

Embodiment 2

[0010] Other conditions are the same as in Example 1, pH is 6, the mass volume concentration of papain is 1%, the water bath temperature is 55° C., and the water bath time is 150 min.

Embodiment 3

[0012] Other conditions are the same as in Example 1, the pH is 7, the mass volume concentration of papain is 2%, the water bath temperature is 50° C., and the water bath time is 150 min.

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PUM

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Abstract

The invention discloses a production process for separating and extracting subprostrate sophora polysaccharide by an enzymatic hydrolysis method, comprising the following steps: A) adding water for soaking for 0.5h based on a mass-to-volume ratio of 1:15; B) adjusting pH value of the soak solution to be 5.0-7.0. adding papain to enable the mass volume concentration to be 1-4%, placing in water bath at the temperature of 50-60 DEG C for reacting for 120-180min, filtering, adding water in residual according to the mass-to-volume ratio of 1:15, placing in boiling water for water bath and filtering; C) merging twice filtrate, distilling and concentrating, adding 95% ethanol to enable the alcohol concentration to reach 80%, pouring supernate out, and centrifuging to obtain precipitate; D) taking precipitate, adding water therein based on the mass-to-volume ratio of 1:5 to dissolve, standing and centrifuging; E) taking supernate, adding 95% ethanol to precipitate, centrifuging, adding 95% ethanol in precipitate and stirring, centrifuging to remove ethanol to obtain the subprostrate sophora polysaccharide wet product; and F) freezing and drying to obtain a dry product. The invention can obviously improve the extraction ratio and total sugar content of the subprostrate sophora polysaccharide.

Description

technical field [0001] The invention relates to a production process for separating and extracting polysaccharide from bean root by enzymatic hydrolysis. Background technique [0002] Sophora subprosrate polysaccharide, as a vaccine immune enhancer for pigs, can significantly improve the protection rate and disease resistance of vaccines, and has broad application prospects. However, the current water-extraction-alcohol-precipitation method for separating and extracting polysaccharides from bean root has the problems of low polysaccharide yield and low total sugar content, resulting in low production efficiency and insufficient supply, making it difficult to meet market demand. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a production process for separating and extracting polysaccharides from bean root by enzymatic hydrolysis with high yield of crude polysaccharides and high total sugar content. [0004] In o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 胡庭俊孙蓉苏子杰曾芸韦英益
Owner GUANGXI UNIV