Application of microRNA-21 in identifying pancreatic cancer

1. microrna-21, pancreatic cancer technology, applied in the field of differential diagnosis of pancreatic cancer, to achieve the effect of high accuracy, good sensitivity, and convenient detection

Inactive Publication Date: 2010-11-10
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the detection of microRNA expression level in serum is a non-invasive and cheap method that is easily accepted by patient

Method used

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  • Application of microRNA-21 in identifying pancreatic cancer
  • Application of microRNA-21 in identifying pancreatic cancer
  • Application of microRNA-21 in identifying pancreatic cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Making a standard map of expression levels of MicroRNA-21 in pancreatic cancer, chronic pancreatitis and normal human plasma

[0021] 1. Plasma collection:

[0022] 31 cases of pancreatic cancer, 14 cases of chronic pancreatitis and 10 normal individuals were selected, and 1ml of whole blood was collected from each case, and centrifuged at room temperature for 1200g×10 minutes and 15000g×10 minutes respectively within 2 hours to obtain plasma.

[0023] 2. RNA extraction from plasma:

[0024] Take 50μl plasma from each individual, add 750μl TRI REAGENT BD ( Molecular research center company) and 20 μl 5N acetic acid, after mixing, add 5 μl of Cel-miR-39 with a concentration of 50 pmol / L, shake and mix, let stand at room temperature for 5 minutes, add 200 μl of chloroform, shake and mix, stand at room temperature for 5 minutes, Centrifuge at 12000g for 15min at 4°C, extract the supernatant, add 4μl DNA mate (TAKARA Company) , then add isopropanol equal ...

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Abstract

The invention discloses a method for identifying pancreatic cancer by taking microRNA in blood as a marker, relating to the technical field of medical molecular biology. The method comprises the following steps of: manufacturing a standard expression level map of microRNA-21 in the pancreatic cancer, chronic pancreatitis and the blood plasma of a normal person through large sample survey and detection; then contrasting the expression abundance value delta Ct of the microRNA-21 in an unknown blood plasma specimen with the standard expression level map of the microRNA-21; if the expression abundance value is within the range of the microRNA of a pancreatic cancer group, judging to be the pancreatic cancer, and if the expression abundance value is within the range of the chronic pancreatitis or the normal person group, basically ruling out the pancreatic cancer. The invention has the characteristics of convenient detection, high accuracy and the like.

Description

technical field [0001] The invention relates to the technical field of medical molecular biology, and relates to a method for identifying pancreatic cancer by using microRNA in blood as a marker. Background technique [0002] MicroRNA, first discovered in 1993, is a small, non-protein-coding single-stranded RNA consisting of 18 to 25 nucleotides. MicroRNA achieves its biological function by binding to the 3'untranslated region (3'UTR) of mRNA, degrading the target mRNA or inhibiting its translation. After more than 20 years of hard work, scientists have made a breakthrough in the research of microRNA, and found that it is ubiquitous in animals, plants and viruses, and plays a very important role in many life activities. According to the latest microRNA database statistics (miRBase Registry 13.0), 706 microRNAs have been found in the human body. microRNA is highly conserved, time-sequential and tissue-specific. [0003] Recent studies have shown that some microRNAs play an...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 李兆申荆清孔祥毓杜奕奇王国坤高军林寒
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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