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Stretch-electricity combinational stimulation cell culture device

A cell culture and cell stimulation technology, applied in the field of cell engineering and tissue engineering, can solve the problems of inability to simulate cells in the body, achieve the effect of improving cell growth conditions, promoting cell growth, and simple operation

Inactive Publication Date: 2010-11-24
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problem that the existing methods cannot simulate the physical microenvironment in which the cells in the body are in tension and electrical stimulation at the same time, and propose a stretch-electric combined stimulation cell culture device, which uses tension stimulation and electrical stimulation Combined loading, the cells are placed under the combined action of stretch stimulation and electrical stimulation, simulating the living environment of cells in vivo, promoting cell growth and differentiation, and constructing cells or tissues with specific functions

Method used

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  • Stretch-electricity combinational stimulation cell culture device

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Under sterile conditions, the cardiomyocytes of 1-3-day-old SD rat suckling mice were divided into 1 × 10 5 / cm 2 The elastic film 305 is densely seeded on the elastic film 305, and the elastic film 305 adopts a silicone rubber film. After pre-culturing for 3 days, the elastic film 305 seeded with cardiomyocytes is placed on the "C"-shaped main structure 309 of the fixed fixture 304 and the sliding fixture 306. , the pressing sheet 310 is wrapped with a layer of stainless steel metal mesh electrode 501 , and then the pressing sheet 310 is placed on the elastic film 305 , and the pressing sheet 310 is pressed with screws 311 to fix the elastic film 305 . Place the fixing fixture 304 and the sliding fixture 308 with the elastic membrane 305 fixed in the tank body 303 of the cell culture chamber 3 , the fixing fixture 304 is placed on the left, and the sliding fixture 308 is placed on the right. The cell culture chamber 3 is then put into the cell culture chamber fixing f...

Embodiment 2

[0054] Press 1 × 10 smooth muscle cells under sterile conditions 5 / cm 2 The elastic film 305 is densely seeded on the elastic film 305, and the elastic film 305 adopts a polylactic acid film. On the second day after the cells adhere to the wall, the elastic film 305 seeded with human smooth muscle cells is placed on the "C"-shaped main body of the fixed fixture 304 and the sliding fixture 306. On the structure 309 , the pressing sheet 310 is wrapped with a layer of platinum mesh electrodes 501 , and then the pressing sheet 310 is placed on the elastic membrane 305 , and the pressing sheet 310 is pressed with screws 311 to fix the elastic membrane 305 . Place the fixing fixture 304 and the sliding fixture 308 with the elastic membrane 305 fixed in the tank body 303 of the cell culture chamber 3 , the fixing fixture 304 is placed on the left, and the sliding fixture 308 is placed on the right. The cell culture chamber 3 is then put into the cell culture chamber fixing frame 2 ...

Embodiment 3

[0058] Press 1 × 10 skeletal muscle cells under sterile conditions 5 / cm 2 The density is seeded on the elastic film 305, and the elastic film 305 is made of a degradable polymer material, such as a random copolymer (PLGA) copolymerized by polylactic acid and polyglycolic acid in a ratio of 90-10:10-90, and the cells adhere to the wall. The next day, the PLGA membrane seeded with skeletal muscle cells was placed on the "C"-shaped main structure 309 of the fixed fixture 304 and the sliding fixture 306, and the pressing sheet 310 was wrapped with a layer of stainless steel metal mesh electrodes 501, and then the pressing sheet 310 was placed On the PLGA film, the pressing piece 310 is pressed with screws 311 to fix the PLGA film. Place the fixing fixture 304 and the sliding fixture 308 with the PLGA membrane fixed in the tank body 303 of the cell culture chamber tank 3, the fixing fixture 304 is placed on the left, and the sliding fixture 308 is placed on the right. The cell c...

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Abstract

The invention discloses a stretch-electricity combinational stimulation cell culture device. The device comprises a cell culture box, a cell culture cavity fixing frame, a cell culture cavity, a stepper motor and a signal generator, wherein the culture box is provided with a porous channel communicated with outside; the cell culture cavity is fixed in the cell culture cavity fixing frame; the cell culture cavity comprises a fixed threaded rod, a groove body, a fixed clamping apparatus, an elastic membrane, a sliding clamping apparatus and a sliding threaded rod; the elastic membrane is fixed on the fixed clamping apparatus and the sliding clamping apparatus; the fixed clamping apparatus is connected with the fixed threaded rod; the sliding clamping apparatus is connected with the sliding threaded rod; the fixed clamping apparatus, the elastic membrane and the sliding clamping apparatus are positioned in the groove body; the sliding threaded rod is connected with the stepper motor; andthe signal generator is connected with a metal electrode in the cell culture cavity. The stretch-electricity combinational stimulation cell culture device overcomes the disadvantage of incapacity of simulating a physical microenvironment in which internal cells are subject to stretching force and electric stimulation at the same time in the conventional method, establishes a cell culture method under the condition of combining stretching force stimulation with electricity stimulation, and promotes the development of cell and tissue culture methods and related tissue engineering reactors.

Description

technical field [0001] The invention relates to a stretch-electric combined stimulation cell culture device, which belongs to the technical field of cell engineering and tissue engineering. Background technique [0002] In tissue engineering, the in vitro functional culture of cells and tissues has become the core of tissue engineering and the necessary technical basis for the formation of the tissue engineering industry. Providing an in vitro growth environment similar to the in vivo living conditions of cells is crucial for the three-dimensional culture and functionalization of cells and tissues, in which physical factors such as mechanics and electricity play a non-negligible role. [0003] The mechanical environment has an important impact on the life activities at all levels, such as organs, tissues, cells and even sub-cells. The regulation of mechanical environment plays a key role in the process of cell and tissue engineering culture, and exerting a certain tension o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42
CPCC12M35/02C12M35/04
Inventor 樊瑜波李萍贾潇凌黄艳徐欢
Owner BEIHANG UNIV
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