Unlock instant, AI-driven research and patent intelligence for your innovation.

Complex microbial agent, preparation method thereof and application thereof in improving yield of crude oil

A complex microbial agent and complex technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex production processes of bacteria, and achieve the effects of low cost, simplified preparation process, and easy production

Active Publication Date: 2013-06-05
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of the complex bacterial production process in the existing method of using anaerobic microorganisms to increase crude oil production, to provide a simplified composite microbial bacterial agent and its preparation method and its application in oil recovery

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In a 500ml serum bottle, add 400ml complex medium (5g / L peptone; 5g / L yeast extract; 2g / L KCl; 1 / L K 2 HPO 4 molasses of 10g / L; pH7.0), with the inoculum size of 1% (weight / weight), insert geobacillus (ATCC51176) and thermophilic anaerobic bacillus (DSM15242), put 60 ℃ of static cultures for 48 hours, Get the microbial agent.

Embodiment 2

[0024] In Daqing Oilfield, 4 low-yield wells were selected to carry out microbial single-well huff and puff tests. The casing addition method was used to inject the microbial agent obtained in Example 1 into the annular space of the pumping casing, and the wells were shut down for 7 days and then resumed. Production, the daily oil production has risen from 2.2 tons / day to 3.0 tons / day, and the effect of oil increase is obvious. The above results show that the oil displacement effect of the provided microbial agent is obvious and the preparation method is simple.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a complex microbial agent and a preparation method thereof, which is used for a microbial oil recovery technology in middle high temperature oil reservoirs. The complex microbial agent mainly comprises aerobic surfactant producing bacteria, anaerobic zymophyte and the like. The complex microbial agent is prepared by introducing the complex microbial bacteria to a culture medium in a natural condition, and culturing the bacteria to proliferate. Due to the adoption of a multiple bacterial combination mode for microbial underground fermentation to increase oil production, the deficiency of environmental impact intolerance of single bacterium can be avoided. Moreover, the complex microbial agent has the advantages of simple preparation and low cost, no antagonistic phenomenon exists among bacterial strains, and the complex microbial agent has the functions of oil displacement and compounding. Thus, the preparation process of a microbial agent for oil recovery is simplified, and the technical effect of the microbial enhanced oil recovery technology is improved greatly.

Description

technical field [0001] The invention relates to a composite microbial bacterial agent, a preparation method thereof, and an application of the composite microbial bacterial agent in increasing crude oil output. Background technique [0002] With the rapid development of our country's economy, the demand for oil is increasing day by day, and the shortage of oil is becoming more and more serious. However, the existing oil extraction technology can only extract about 1 / 3-1 / 2 of the reserves, and the remaining recoverable oil reserves are huge. How to extract these remaining oil resources is a problem that people pay close attention to. [0003] Microbial Enhanced Oil Recovery (MEOR) is a biotechnology that uses the life activities of microorganisms in the oil reservoir and their metabolites to increase crude oil production. It has the characteristics of wide application range, low implementation cost, and significant economic benefits. has received more and more people's atten...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/01
Inventor 薛燕芬马延和马树恒袁三青
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI