Reverse dot blot (RDB) detection membrane strip, detection method and kit for identifying insects

A technology of insects and membrane strips, applied in the field of insect identification, can solve problems such as the application of RDB technology that has not been seen

Inactive Publication Date: 2010-12-15
SHENZHEN AUDAQUE DATA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no application of RDB technology in insect species identification so far.

Method used

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  • Reverse dot blot (RDB) detection membrane strip, detection method and kit for identifying insects
  • Reverse dot blot (RDB) detection membrane strip, detection method and kit for identifying insects
  • Reverse dot blot (RDB) detection membrane strip, detection method and kit for identifying insects

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0110] (1) RDB detection film strips for identification of 21 Coleopteran insects

[0111] The 21 species of Coleoptera are:

[0112] Heterobostrychus aequalis (Waterhouse) (Insect 1 for short);

[0113] Heterobostrychus brunneus (Murray) (insect 2 for short);

[0114] Heterobostrychus hamatipennis Lesne (insect 3 for short);

[0115] Sinoxylon conigerum Gerstoker (insect 4 for short);

[0116] Sinoxylon anale Lsene (insect 5 for short);

[0117] Sinoxylon ruficome Fahraus (insect 6 for short);

[0118] Sinoxylon senegalense Karsch (insect 7 for short);

[0119] Xylothrips flavipes (Illiger) (insect 8 for short);

[0120] Dinoderus minutus (Lesne) (insect 9 for short);

[0121] Medium-to-long bark beetle Platypus parallelus (Fabricius) (referred to as insect 10);

[0122] Platypus quercivorus Murayama (insect 11 for short);

[0123] Treptoplatypus solidus Walk (insect 12 for short);

[0124] Scattered pair of long bark beetle Platypus solutus Schedl (referred to as i...

specific Embodiment approach 2

[0239]RDB Kit for the Identification of 21 Coleopteran Insects Infecting Wood

[0240] The kit of this specific embodiment includes RDB detection membrane strips, universal primers, positive control plasmids, POD, TMB, H 2 o 2 As well as the instruction manual, see Table 6 for the quantity or volume and storage conditions of each component.

[0241] Table 6

[0242] contents

[0243] Positive control sample (P)

[0244] The detection kit can realize the identification of 21 kinds of Coleoptera insects in the first embodiment. It has fast, sensitive and multi-species parallel detection capabilities, and the detection results are stable and reliable. It can be applied to the detection and monitoring of such insects in the entry-exit inspection and quarantine system and agricultural plant protection departments, and can also be used in the teaching of insect species identification or distinction. Experiment and research.

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Abstract

The invention relates to a reverse dot blot (RDB) detection membrane strip, a detection method and a kit for identifying insects. The membrane strip comprises a universal primer, a solid phase carrier, a probe and a PCR positive quality control plasmid, wherein the probe is fixed on the carrier; the universal primer is a degenerate primer pair TF / TR; the solid phase carrier is a Biodyne C membrane; the probe fixed on the carrier is a group of arrays consisting of a quality control probe and an RDB detection probe; the quality control probe comprises a color development point probe, a positive comparison probe and a negative comparison probe; and the RDB detection probe is a section of 15 to 19nt oligonucleotide of which the 5' end is marked with amino (-NH2). The membrane strip can rapidly, correctly and vividly realize correct classified identification of adults, larvae, eggs, pupae and the like of insects and solves the problems of the need of professional classification knowledge, type specimen comparison, a large number of insect types and difficult identification existing in the conventional morphological insect identification, particularly the problem that the larvae, the eggs and the pupae of the insects and the adults of which the identification characteristics are damaged cannot be identified effectively.

Description

technical field [0001] The invention relates to insect identification, in particular to an RDB detection membrane strip, a detection method and a kit for insect identification. Background technique [0002] Insects are the largest group of invertebrates in the animal kingdom Arthropoda, with more than 1 million species described in the world. The identification of insect species generally adopts the traditional morphological identification method, which classifies and identifies insects by summarizing their common and characteristic biological traits. The morphological identification of insect species is mainly through the morphological characteristics of adults or larvae. There is no internationally recognized standard and fixed model for the extraction and selection of morphological characteristics for classification, and there is not even a completely unified insect morphological classification system. Deoxyribose nucleic acid (Deoxyribonucleic acid, abbreviated as DNA),...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 余道坚徐浪焦懿陈志粦郑耘康林王银竹陈乃中徐孟杰胡斌
Owner SHENZHEN AUDAQUE DATA TECH
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