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A kind of rdb detection membrane strip, detection method and kit for insect identification

A technology for insects and membrane strips, applied in the field of insect identification, can solve problems such as the application of RDB technology that has not been seen

Inactive Publication Date: 2016-07-06
SHENZHEN AUDAQUE DATA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no application of RDB technology in insect species identification so far.

Method used

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  • A kind of rdb detection membrane strip, detection method and kit for insect identification
  • A kind of rdb detection membrane strip, detection method and kit for insect identification
  • A kind of rdb detection membrane strip, detection method and kit for insect identification

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Experimental program
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Effect test

specific Embodiment approach 1

[0110] (1) RDB detection film strips for identification of 21 Coleopteran insects

[0111] The 21 species of Coleoptera are:

[0112] Heterobostrychusaequalis (Waterhouse) (Insect 1 for short);

[0113] Heterobostrychusbrunneus (Murray) (insect 2 for short);

[0114] Heterobostrychushamatipennis Lesne (insect 3 for short);

[0115] Sinoxylonconigerum Gerstoker (insect 4 for short);

[0116] Sinoxylonanale Lsene (insect 5 for short);

[0117] Sinoxylonruficome Fahraus (insect 6 for short);

[0118] Sinoxylonsenegalense Karsch (insect 7 for short);

[0119] Xylothripsflavipes (Illiger) (Insect 8 for short);

[0120] Dinoderus minutus (Lesne) (insect 9 for short);

[0121] Medium-to-long beetle Platypusparallelus (Fabricius) (insect 10 for short);

[0122] Platypusquercivorus Murayama (insect 11 for short);

[0123] Treptoplatypus solidus Walk (insect 12 for short);

[0124] Scattered pair of long bark beetle Platypussolutus Schedl (referred to as insect 13);

[0125] ...

specific Embodiment approach 2

[0239] RDB Kit for the Identification of 21 Coleopteran Insects Infecting Wood

[0240] The kit of this specific embodiment includes RDB detection membrane strips, universal primers, positive control plasmids, POD, TMB, H 2 o 2 As well as the instruction manual, see Table 6 for the quantity or volume and storage conditions of each component.

[0241] Table 6

[0242] contents

[0243] The detection kit can realize the identification of 21 kinds of Coleoptera insects in the first embodiment. It has fast, sensitive and multi-species parallel detection capabilities, and the detection results are stable and reliable. It can be applied to the detection and monitoring of such insects in the entry-exit inspection and quarantine system and agricultural plant protection departments, and can also be used in the teaching of insect species identification or distinction. Experiment and research.

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Abstract

An RDB detection membrane strip, detection method and kit for insect identification, the membrane strip includes a universal primer, a solid phase carrier, a probe immobilized on the carrier and a PCR positive quality control plasmid, and the universal primer is a degenerate primer pair TF / TR, the solid phase carrier is Biodyne C membrane, and the probes immobilized on the carrier are an array composed of a set of quality control probes and RDB detection probes. Quality control probes include chromogenic point probes, positive control probes and negative control probes. The RDB detection probes are all oligonucleotides of 15-19 nt, and the 5' ends are labeled with amino groups (-NH2). It can quickly, accurately and intuitively classify and identify adults, larvae, eggs, and pupae of insects, and solve the problems of traditional morphological insect identification that require professional taxonomic knowledge, comparison of type specimens, and many types of insects, making identification difficult. It is difficult to effectively identify insect larvae, eggs, pupae, and adult insects with damaged identification features.

Description

technical field [0001] The invention relates to insect identification, in particular to an RDB detection membrane strip, a detection method and a kit for insect identification. Background technique [0002] Insects are the largest group of invertebrates in the animal kingdom Arthropoda, with more than 1 million species described in the world. The identification of insect species generally adopts the traditional morphological identification method, which classifies and identifies insects by summarizing their common and characteristic biological traits. The morphological identification of insect species is mainly through the morphological characteristics of adults or larvae. There is no internationally recognized standard and fixed model for the extraction and selection of morphological characteristics for classification, and there is not even a completely unified insect morphological classification system. Deoxyribose nucleic acid (Deoxyribonucleic acid, abbreviated as DNA),...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 余道坚徐浪焦懿陈志粦郑耘康林王银竹陈乃中徐孟杰胡斌
Owner SHENZHEN AUDAQUE DATA TECH
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