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Method for cultivation of microorganisms of order thraustochytriales

A thraustochytrid and microorganism technology, applied in fungi, fermentation and other directions, can solve the problems of complex production steps, unsatisfactory lipid yield and the like

Inactive Publication Date: 2014-03-12
LONZA LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Current methods for the production of DHA and / or DPA by culturing Ulkenia lead to unsatisfactory lipid yields, especially low yields of DHA and / or DPA or involve complicated production steps

Method used

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  • Method for cultivation of microorganisms of order thraustochytriales
  • Method for cultivation of microorganisms of order thraustochytriales
  • Method for cultivation of microorganisms of order thraustochytriales

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: the cell culture medium (medium A) containing ammonium tartrate:

[0067]

Embodiment 2

[0068] Embodiment 2: Cell culture medium (medium B) containing ammonium chloride:

[0069]

Embodiment 3

[0070] Example 3: Culture of Thraustochytriales microorganisms

[0071] Using two different cell culture media (medium A and B) of the present invention to cultivate Thraustochytrides microorganisms compared with the prior art (medium C), the same kind of microorganisms were cultivated in the cell culture medium. The amounts of ammonium salts (ammonium tartrate and ammonium chloride) used in media A and B yielded the same amount of ammonium ions in media C.

[0072] As the microbial cell, Ulkenia sp. SAM2179 was used.

[0073] For the production of PUFAs, microorganisms are cultured according to the following method:

[0074] 1. Preparatory training:

[0075] The following preliminary cultures were used in all 3 media:

[0076] Glucose (g / L)30,0

[0077] Yeast extract (g / L) 10,0 (Sensient)

[0078] Tropic Marin (g / L) 16,65

[0079] (Dr. Bienert GmbH, Wartenberg)

[0080] The pH was adjusted to 6.0 using HCl.

[0081] Microorganisms were cultured in a 2-liter Erlenmeyer...

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Abstract

The present invention relates generally to the field of microorganism cell culture. It devises a cell culture medium for and a method of culturing microorganisms of the order Thraustochytriales, especially for the production of omega-3-fatty acids. Furthermore it devises the use of ammonium tartrate as nitrogen source for the cultivation of microorganisms of the order Thraustochytriales.

Description

technical field [0001] The present invention relates generally to the field of microbial cell culture. A cell culture medium for microorganisms of the order Thraustochytriales and a method for culturing microorganisms of the order Thraustochytriales have been invented, especially for the production of omega-3 fatty acids. Furthermore, the use of ammonium tartrate as a nitrogen source for the cultivation of Thraustochytriidea was invented. Background technique [0002] The present invention discloses a method for increasing the total amount of PUFA in the biomass of Thraustochytrides microorganisms by improving the cell culture medium, especially a method for increasing the total amount of ω-3 docosahexaenoic acid (DHA). The amount of DHA in the biomass was increased when specific salts, especially ammonium tartrate, were present in the cell culture medium. Since not only the total amount of DHA is increased, but also the total amount of lipids is increased, the production ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/64
CPCC12N1/14C12P7/6427
Inventor 马库斯·卢伊
Owner LONZA LTD
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