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Portable reagent quantitative detection system based on informationized platform, application and method thereof

A quantitative detection method and quantitative detection technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of lagging inspection level and disease diagnosis level, medical units can not carry out testing, and can not be widely carried out.

Active Publication Date: 2011-01-05
WUHAN EASYDIAGNOSIS BIOMEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of test has great limitations, and it cannot be widely carried out due to the constraints of supporting equipment. Performed in the laboratory, not at the bedside
For grassroots and community hospitals in China, many routine testing items cannot be carried out due to lack of financial resources to purchase quantitative testing equipment, and the level of testing and disease diagnosis is quite lagging behind. This is where the gap between our medical level and developed countries lies.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Quantitative detection of D-dimer in human whole blood (or serum / plasma)

[0029] 1) Establishment of standard curve for each batch of D-dimer detection kit (colloidal gold method): each batch of products is tested with D-dimer standard substances of different concentrations, and the gray level of a detection line is obtained for each concentration value value, that is, to establish a standard curve for this batch of products. The standard curve information is compressed into the product code of each independent reagent in the batch, and the standard curve information can be obtained by identifying the product code.

[0030] 2) Adding samples at the detection terminal: Use the D-dimer detection kit (colloidal gold method) to detect the D-dimer concentration in human whole blood (or serum / plasma), take 120 μl of whole blood and add it to the kit. After the sample hole, the reaction area of ​​the reagent kit is inserted into the socket of a miniature image sca...

Embodiment 2

[0034] Embodiment 2: Quantitative detection of luteinizing hormone (LH) in human urine

[0035] 1) Standard curve establishment for each batch of Luteinizing Hormone (LH) Detection Kit (Colloidal Gold Method): Each batch of products is tested with LH standard substances of different concentrations, and each concentration value obtains a gray value of a detection line, namely A standard curve for this batch of products was established. The standard curve information is compressed into the product code of each independent reagent in the batch, and the standard curve information can be obtained by scanning the product code.

[0036] 2) Detection by adding samples at the detection terminal: detecting the LH concentration in human urine with an LH detection kit (colloidal gold method). Insert the reagent strip into the urine for 30 seconds, and then place it flat on a flat table. After 5 minutes, the user directly takes a photo of the detection area with a mobile phone, edits a sp...

Embodiment 3

[0040] Embodiment 3: Quantitative detection of N-terminal BNP precursor in human whole blood (or serum / plasma)

[0041] 1) Establishment of standard curve for each batch of N-terminal pro-brain natriuretic peptide (NT-proBNP) detection kit (immunofluorescence method): each batch of products is detected with different concentrations of NT-proBNP, and one detection is obtained for each concentration value The fluorescence intensity value of the line is to establish the standard curve of the batch of products. The standard curve information is compressed into the product code of each independent reagent in the batch, and the standard curve information can be obtained by scanning the product code.

[0042] 2) Adding samples to the detection terminal: use the N-terminal pro-BNP detection kit (immunofluorescence method) to detect the NT-proBNP concentration in human whole blood (or serum / plasma), take 120 μl of whole blood, and add it to the kit Insert the reaction area of ​​the ki...

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PUM

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Abstract

The invention relates to a portable reagent quantitative detection system based on an informationized platform, application and a method thereof. The portable reagent quantitative detection system comprises a detection reagent and an informationized platform, wherein the informationized platform comprises a plurality of detection terminals and a center end, the detection reagent has a product information code which can be identified by the informationized platform, and the detection terminals are connected with the center end by a wireless or wire communication mode. In the portable reagent quantitative detection system and the method, the detection terminals only need to be matched with a camera mobile phone or a micro-image acquisition instrument, and the cost is only about 100 yuan. Owing to a portability characteristic, the portable reagent quantitative detection system can be used for detecting at all times and places, is convenient to develop in families or medical institutions at all levels and complies with a proponent for developing new healthcare reform pharmaceutical industry of China to a basic level very much.

Description

technical field [0001] The invention relates to an information platform-based portable reagent quantitative detection system and its application and method. Through the detection terminal and the central terminal information platform, the reagent quantitative detection can be realized at any time and in multiple places. Background technique [0002] The current quantitative detection of reagents in China is completed through reagents and supporting quantitative detection equipment, that is, each testing unit needs to be equipped with quantitative detection equipment, and the quantitative detection is completed by adding samples and then on the machine. This detection mode is widely used in clinical and Laboratory testing. However, this kind of test has great limitations, and it cannot be widely carried out due to the constraints of supporting equipment. It is performed in the laboratory, not at the bedside. For grassroots and community hospitals in China, many routine test...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/53
Inventor 陈莉莉王颖
Owner WUHAN EASYDIAGNOSIS BIOMEDICINE
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