Serum polypeptide internal standard correction detection method
A serum polypeptide and detection method technology, applied in the direction of measuring devices, material analysis through electromagnetic means, instruments, etc., to achieve the effect of peak accuracy and avoid drift
Inactive Publication Date: 2011-02-02
毅新兴业(北京)科技有限公司
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[0005] So far, there is no relevant report on the technology of effective internal standard correction for the detection of serum peptides
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Embodiment 1
[0020] In this example, the human serum was treated with SPE-C magnetic beads, and the spectrum obtained by MALDI-TOF was the serum peptide fingerprint, referred to as the blood peptide spectrum. The purpose of adding an internal standard to the enrichment solution treated with SPE-C magnetic beads is that when MALDI-TOF linearly collects data, after the external standard is corrected, it is calibrated again with the internal standard to make each peak in the blood peptide spectrum more accurate and avoid molecular weight. drift.
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The invention provides a method for detecting serum polypeptide by internal standard correction. In the method, polypeptide with known protein peak is added into a serum sample, the sample is detected by a mass spectrum, and the chromatogram of the serum is corrected according to the protein peak of the polypeptide. The method can correct and eliminate the influence on analysis results due to fluctuation of operating conditions and improve the accuracy of the analysis results. When data is linearly acquired by an MALDI-TOF, after an external standard performs correction, the internal standard performs correction again, so that each peak in a serum polypeptide spectrum is more accurate and the shift of molecular weight is avoided.
Description
technical field [0001] The invention relates to the detection of serum polypeptides, in particular to a method for calibrating and detecting serum polypeptides using mass spectrometry. Background technique [0002] Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) technology is one of the rapidly developing proteomics technologies in recent years, with high sensitivity, high accuracy and With the characteristics of high resolution, it provides fast and high-quality analysis and testing methods for life science research and clinical major disease early warning and auxiliary diagnosis, and is also a good molecular marker method for screening diseases. [0003] Most of the current related research is the application of multi-dimensional chromatographic separation combined with electrospray ionization source (ESI source) mass spectrometry, but for matrix-assisted laser desorption ionization (MALDI) source mass spectrometry, due to the de...
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Inventor 田亚平马庆伟李燕刘丽华张燕任晶
Owner 毅新兴业(北京)科技有限公司