Method for improving spore germination rate of beauveria bassiana by adding nutrients
A technology of Beauveria bassiana and spore germination rate, which is applied in the direction of using spores, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of enhancing virulence, increasing spore germination rate, and improving germination rate
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[0007] Preparation of spore suspension:
[0008] 1. Elution. The bacterial classification used in the present invention is Beauveria bassiana Beauveria bassiana (preservation number is CCTCC No: M209238 ), take an appropriate amount of culture culture that is fresh or stored within 3 months (preferably preserved) in a refrigerator at 4°C in a 250 mL Erlenmeyer flask, and add an appropriate amount of 0.05% Tween- 80 solution and glass beads, crush the culture with a glass rod, and shake it on a shaker at 25°C and 180rpm for 30min (above).
[0009] 2. Filter. After shaking, take out the triangular flask, filter with 2-4 layers of gauze, and take the filtrate.
[0010] 3. Centrifugal. The filtrate was centrifuged under the following conditions: 8000 rpm, 5 min, 4°C, and the supernatant was discarded.
[0011] 4. Reconstitution. The centrifuged precipitate was redissolved by adding a certain amount of 0.05% Tween-80 solution, stirred evenly, and prepared into a spore suspen...
Embodiment 1
[0019] Example 1: The strategy of adding nutrients to the spore suspension was adopted. The addition method was to mix the spore suspension with the bran extract with a concentration of 1% in a volume ratio of 1:1, and the rest of the conditions were the same as in the comparative example. Then the final concentration of spores in the reaction liquid is 2×10 7 Spores / mL, the final concentration of bran extract in the reaction solution was 0.5%. Results: The germination rate of Beauveria bassiana spores was 65.59%.
Embodiment 2
[0020] Example 2: The strategy of adding nutrients to the spore suspension is adopted. The addition method is to mix the spore suspension with the bran extract with a concentration of 10% in a volume ratio of 1:1, and the remaining conditions are the same as in the comparative example. Then the final concentration of spores in the reaction liquid is 2×10 7 Spores / mL, the final concentration of bran extract in the reaction solution was 5%. Results: The germination rate of Beauveria bassiana spores was 83.05%.
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