Method for separating rare actinomycete

Abstract

The invention discloses a method for separating rare actinomycete, belongs to the technical field of microorganisms, and is mainly used for separating the rare actinomycete from soil. The method solves the problem of interference of dominant microflora in the soil during separation of the rare actinomycete, and the dominant microflora in a soil sample is subjected to elutriation by sterile water for multiple times and is reduced. According to a characteristic that the actinomycete is easily attached to a nutrient medium, sediment sludge remained after the elutriation is inoculated into an actinomycete selective medium to improve the success rate of separating the rare actinomycete.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms and is applied to the separation of rare actinomycetes. Background technique [0002] Actinomycete is an important source of antibiotics, enzymes, and enzyme inhibitors. Rare actinomycete refers to the occurrence of some species that are much lower than common Streptomyces when samples are separated by conventional separation procedures and separation methods. Taxa. Some scholars classify all non-streptomyces as rare actinomycetes. The main isolation source of actinomycetes is soil, streptomyces is its dominant flora, and the distribution density of non-streptomyces is relatively low. [0003] So far, the discovery of many known metabolites of commercial significance has almost all relied on the use of new screening systems and new microbial isolation and identification techniques. However, due to the long-term use of traditional strain isolation and identification techniques in the screening p...

AI Technical Summary

Problems solved by technology

The dilution method is only suitable for the separation of microorganisms or their propagules that are easy to enter the aqueous solution, so it is particularly biased towards polyspore-producing fungi, single-celled bacteria, motile flagella, and dominant flora in the soil, and some actinomycetes The rhizoids produced by the bacteria are easy to grow into the nutrient matrix, not easy to separate from the soil particles, and not easy to produce asexual spores, so it is not easy to enter the solution, and it is difficult to separate this kind of actinomycetes by dilution method

If the soil sample is directly inoculated on the actinomycetes selection medium without washing with sterile water, it will be easily interfered by other dominant actinomycetes, even bacteria and fungi, making it difficult to separate