Phytoene desaturase gene of sphingomonas sp. and application thereof

A technology of phytoene and monas, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc.
CN101979587BInactive Publication Date: 2013-05-01ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2013-05-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a DNA sequence of a phytoene desaturase gene of sphingomonas sp., a recombinant strain with phytoene desaturase lost and application thereof. The phytoene desaturase gene (crtI) of the sphingomonas sp. has a nucleotide sequence shown by SEQ ID No.1. The DNA sequence of the phytoene desaturase gene (crtI) of the sphingomonas sp. provided by the invention lays a foundation for the genetic transformation of a biological synthesis manner of sphingomonas sp. carotene. Moreover, compared with the wild strains, the sphingomonas sp. recombinant strain with the phytoene desaturase lost, which is constructed by a gene knockout method, has gellan glue yield unchanged, generates no uranidin, reduces the amount of ethanol or isopropanol which is used during gellan glue extraction and therefore reduces the production cost of the gellan glue; meanwhile, the strain can also be applied to the further gene knockout or metabolic engineering transformation.
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Description

(1) Technical field

[0001] The invention relates to the phytoene dehydrogenase gene (crtI) of Sphingomonas paucimobilis and its application. (2) Background technology

[0002] Gellan gum is a new type of microbial exopolysaccharide, which is composed of β-1,3-D-glucose, β-1,4-D-glucuronic acid and α-1,4-L-rhamnose in molar ratio 2:1:1 composition of polymers. Compared with similar products, it has many superior properties, such as less dosage; adjustable freezing point, melting point, elasticity, hardness, etc.; the formed gel has high transparency and strength; good taste performance and high thermal stability. Due to its good properties, gellan gum is widely used in food and pharmaceutical industries as emulsifier, suspending agent, thickener, stabilizer, gelling agent, sustained release agent, film-forming material, etc. In addition, gellan gum can also be compounded with other colloids to give the product a unique taste and flavor, and has broad application prospects. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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