Plant stress resistance related protein as well as coding gene and application thereof
A gene and protein technology, applied in the biological field, can solve the problem that the function of calmodulin-like genes is poorly understood
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0062] Embodiment 1, cultivating reverse-resistant gene Arabidopsis thaliana
[0063] 1. Construction of recombinant expression vector
[0064] 1. Cloning of OsMsr2 gene
[0065] ① Rice total RNA extraction
[0066] Plant tissue material Pei'ai 64s (provided by Luo Xiaohe, National Hybrid Rice Engineering Technology Research Center, the public can obtain it from the germplasm resource bank of Hunan Academy of Agricultural Sciences or Luo Xiaohe, the breeder of Pei'ai 64s), literature: Luo Xiaohe, Qiu Zhizhong, Li Renhua. The dual-purpose GMS line Peiai 64S that leads to a low sterility critical temperature. Hybrid rice, 1992, (1): 27-29) was quickly ground into powder in liquid nitrogen, and about 100 mg of powder material was taken Place in a 1.5ml centrifuge tube filled with 1.0ml TRIzol (Invitrogen), and store at -80°C for later use. Using TRIzol reagent extraction method: Take out the spare sample stored at -80°C, after thawing at room temperature, add 200 μl chloroform...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com