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Plant stress resistance related protein as well as coding gene and application thereof

A gene and protein technology, applied in the biological field, can solve the problem that the function of calmodulin-like genes is poorly understood

Inactive Publication Date: 2011-03-30
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, studies on plant calmodulin-like genes mainly focus on Arabidopsis, and little is known about the functions of calmodulin-like genes in rice.

Method used

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  • Plant stress resistance related protein as well as coding gene and application thereof
  • Plant stress resistance related protein as well as coding gene and application thereof
  • Plant stress resistance related protein as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, cultivating reverse-resistant gene Arabidopsis thaliana

[0063] 1. Construction of recombinant expression vector

[0064] 1. Cloning of OsMsr2 gene

[0065] ① Rice total RNA extraction

[0066] Plant tissue material Pei'ai 64s (provided by Luo Xiaohe, National Hybrid Rice Engineering Technology Research Center, the public can obtain it from the germplasm resource bank of Hunan Academy of Agricultural Sciences or Luo Xiaohe, the breeder of Pei'ai 64s), literature: Luo Xiaohe, Qiu Zhizhong, Li Renhua. The dual-purpose GMS line Peiai 64S that leads to a low sterility critical temperature. Hybrid rice, 1992, (1): 27-29) was quickly ground into powder in liquid nitrogen, and about 100 mg of powder material was taken Place in a 1.5ml centrifuge tube filled with 1.0ml TRIzol (Invitrogen), and store at -80°C for later use. Using TRIzol reagent extraction method: Take out the spare sample stored at -80°C, after thawing at room temperature, add 200 μl chloroform...

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Abstract

The invention discloses a protein which is named as OsMSr2 and is from rice. The protein is (1) a protein formed from an amino acid sequence shown by the sequence 2 in a sequence table or (2) a plant stress resistance related protein derived from the protein (1) by replacing and / or deleting and / or adding one or more than one amino acid from the amino acid sequence shown by the sequence 2 in the sequence table. Introducing the coding gene OsMsr2 of the protein into a plant can increase the germination rate of seeds under adverse conditions, increase root length and root weight, increase the growth potential of the plant, ensure seeds are enlarged, increase the net photosynthetic rate of leaves and increase the tolerance of the plant under adverse conditions.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein related to plant stress tolerance and its coding gene and application. Background technique [0002] Drought, low temperature, and high temperature are common adversity factors affecting crop growth and development, which largely limit crop yield and geographical distribution. In order to improve the yield of crops under stress and break the geographical distribution restrictions, it is an effective way to find new stress-tolerant genes and use genetic engineering to improve crop stress tolerance. Traditional methods are limited due to the complexity of plant stress regulation mechanisms and the lack of effective screening techniques. Studies have shown that most genomes in plants are involved in their own responses to stress factors such as drought and high salinity, and changing the expression of a single gene can significantly improve the ability of plants to resist dro...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N5/10C12N1/00C12N15/63C12N15/66C12N15/82
Inventor 夏新界徐国云
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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