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Bacterial cellulose strain

A technology of bacterial cellulose and bacterial strains, applied in the field of microorganisms, can solve the problems of low yield and instability of bacterial cellulose strains

Inactive Publication Date: 2011-03-30
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the relatively low and unstable defects of bacterial cellulose strains currently reported, the purpose of the present invention is to obtain a high-yield bacterial cellulose new bacterial strain M438 through the isolation and high-pressure mutagenesis of bacterial cellulose strains.

Method used

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Embodiment Construction

[0026] In order to obtain high-yielding and stable bacterial cellulose strains and increase the yield of bacterial cellulose, the present invention uses ultra-high pressure technology to conduct mutagenesis treatment on the screened bacterial cellulose strains on the basis of previous research to obtain high-yield bacterial cellulose The cellulose yield (wet film) of strain M438 can reach 40g / 100mL, while the highest bacterial cellulose yield reported so far does not exceed 30g / 100mL.

[0027] 1) Preparation of bacterial cellulose strain M438:

[0028] This embodiment relates to the bacterial cellulose strain M438 for screening, which is isolated and screened from buckwheat vinegar mash and obtained after high hydrostatic pressure mutagenesis, specifically including the following steps:

[0029] (1) Isolation and purification of bacterial cellulose strains:

[0030] Bacterial cellulose-producing bacteria were isolated from buckwheat vinegar mash brewed in the laboratory. The ...

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Abstract

The invention discloses a new bacterial cellulose strain which is named Gluconacetobacter.sp.M438 and has the Collection number of CGMCC No. 3917 in China General Microbiological Culture Collection Center (CGMCC). The strain is obtained through the processes of separating, selecting and carrying out high-pressure mutagenesis of buckwheat vinegar mashes, is identified as a subspecies of Ga. hansenii, and is the new bacterial cellulose strain, wherein the cellulose yield of the strain can reach up to 40g / 100mL culture medium. The bacterial cellulose strain has the advantage of high and stable yield and was collected in CGMCC on June 10, 2010.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to a bacterial strain, in particular to a bacterial cellulose strain ( Gluconacetobacter. Sp ) isolation, mutagenesis, identification and culture. Background technique [0002] In addition to the ability of plants and animals to synthesize cellulose, several bacteria can produce extracellular cellulose more efficiently than plants in a heterotrophic manner. Cellulose synthesized by microorganisms is usually called "Bio-cellulose" or "Bacterial cellulose (BC)". Bacterial cellulose has many excellent properties, such as high purity, ultrafine, high crystallinity, high water holding capacity, high strength, good biodegradability and controllability of the synthesis process. As a new type of microbial synthetic material, bacterial cellulose has been used in many fields such as food, papermaking, and medicine. [0003] However, the current production of bacterial cellulose-producing ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C12R1/01
Inventor 李志西杜双奎葛含静杨甲平林德彗
Owner NORTHWEST A & F UNIV
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