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Method for preparing harmane alkaloids from fruit residues of hippophae rhamnoides

A technology of sea buckthorn pomace and halman alkali, applied in the direction of organic chemistry and the like, to achieve the effects of simple preparation method and high extraction rate

Inactive Publication Date: 2011-05-04
QINGHAI TSINGHUA BIOTRY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no Harman base extracted from plants, and the extraction of Harmon from seabuckthorn waste residue has not been reported in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Take 100kg of seabuckthorn pomace obtained by centrifugation, crush and sieve, add 80% ethanol to ultrasonic extraction, the material liquid is 1:20 by weight and volume, extraction temperature is 75℃, extraction 3 times, extraction time 72min, concentrated recovery ethanol Obtain the extract, temperature 40℃, pressure 60kPa, add water to disperse the extract, over-adsorb the resin until the adsorption is saturated, wash with deionized water to neutral, and add 20% ethanol for elution, collect the eluate, Let it stand overnight, centrifuge the precipitate, wash with deionized water until it is neutral, dissolve in absolute ethanol, filter, concentrate the filtrate under reduced pressure at a temperature of 80°C and a vacuum of -0.07MPa, and heat the concentrate in a constant temperature drying box It is dried at 50°C for 6 hours to obtain 0.78kg of Halman base.

Embodiment 2

[0012] Take 100kg of seabuckthorn pomace obtained by centrifugation, crush and sieve, add 75% ethanol for ultrasonic extraction, the material liquid is 1:30 by weight and volume, extraction temperature is 50℃, extraction 3 times, extraction time 30min, concentrated recovery ethanol Obtain the extract, temperature 50℃, pressure 70kPa, add water to disperse the extract, over the macroporous adsorption resin, until the adsorption is saturated, wash with deionized water to neutral, and add 25% ethanol for elution, collect the eluate, static Leave it overnight, centrifuge the precipitate, wash with deionized water until it is neutral, dissolve in absolute ethanol, filter, concentrate the filtrate under reduced pressure at a temperature of 70°C and a vacuum of -0.08MPa, and heat the concentrate in a constant temperature drying oven Dry for 5 hours at 60°C to obtain 0.76kg of Halman's base.

Embodiment 3

[0014] Take 100kg of sea-buckthorn pomace after centrifugation, crush and sieve, add 95% ethanol for ultrasonic extraction, the material liquid is 1:40 by weight and volume, extraction temperature is 90℃, extraction 3 times, extraction time 110min, concentrated recovery ethanol Obtain the extract, temperature 60℃, pressure 88kPa, add water to disperse the extract, over the macroporous adsorption resin, until the adsorption is saturated, wash with deionized water to neutral, and add 30% ethanol for elution, collect the eluate, static Let it stand overnight, centrifuge the precipitate, wash with deionized water to neutrality, dissolve in absolute ethanol, filter, concentrate the filtrate under reduced pressure at a temperature of 90°C and a vacuum of -0.06MPa, and heat the concentrate in a constant temperature drying oven Dry for 3 hours at 70°C to obtain 0.77kg of Halman's base.

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PUM

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Abstract

The invention relates to a method for preparing harmane alkaloids from fruit residues of hippophae rhamnoides, which comprises the processes of extracting, concentrating, dispersing, purifying, eluting, centrifuging, dissolving in ethanol, concentrating and drying. The preparation method is simple and contributes to industrial production; the extraction ratio reaches 90 percent; meanwhile, hippophae rhamnoides resources can be comprehensively utilized.

Description

Technical field [0001] The invention relates to a method for preparing halmanine, in particular to a method for preparing halmanine from sea buckthorn pomace. Background technique [0002] Seabuckthorn, also known as Elaeagnus, vinegar willow fruit, black thorn, sour thorn, Qicha Rigana (Mongolian name), Dap (Tibetan name), Jihan (Uyghur name), is a plant of Elaeagnus seabuckthorn ( Hippophae rhamnoides L.). The roots, stems, leaves, flowers and fruits of sea buckthorn are rich in physiologically active substances. In particular, the pomace of sea buckthorn after extracting juice and seeds by centrifugal separation is rich in natural Halmanine, with an average content of 0.85%. [0003] Harmane (Harmane), synonymous demethoxy harmine; 2-methyl-B-carboline; 3-methyl-4-carboline, widely present in Coriolus maximus , Mushrooms and plants, belong to B-carboline alkaloids. The molecular formula is C 12 H 10 N 2 , The molecular weight is 182.22, and the structural formula is as foll...

Claims

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Application Information

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IPC IPC(8): C07D471/04
Inventor 鲁长征山永凯李树志索有瑞任蓓蕾马宁安刘洪智杨莉华李安军年璟远张辉李宁
Owner QINGHAI TSINGHUA BIOTRY BIO TECH
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