Glucose aqueous solution quick identification method based on near infrared spectrum technology

Abstract

The invention relates to a glucose aqueous solution quick identification method based on a near infrared spectrum technology. The method comprises the following steps of: (1) screening a plurality of near infrared spectrum data of glucose aqueous solutions of different concentrations to find out a characteristic absorption peak wavelength value capable of identifying the glucose constituent in the aqueous solutions; (2) collecting the near infrared spectrum data of an unknown aqueous solution sample; (3) dividing the near infrared spectrum data of the unknown aqueous solution sample collected in the steps (2) from the standard near infrared spectrum data of pure water, and then smoothing and carrying out first-order derivative processing to obtain a processed near infrared spectrogram; and (4) judging whether the known aqueous solution is a glucose aqueous solution or not according to the spectrogram processed in the step (3). The glucose aqueous solution identification method can be used for quickly and qualitatively analyzing glucose in an aqueous solution and has the advantages of higher sensitivity and no damage to the sample.

Description

[0001] technical field [0002] The invention relates to a rapid identification and detection method in the field of analytical chemistry, in particular to a rapid identification method for aqueous glucose solution based on near-infrared spectroscopy. Background technique [0003] Currently, the reported methods for qualitative analysis of glucose in aqueous solution include conventional chemical analysis, gas chromatography-mass spectrometry, and high-performance liquid chromatography-mass spectrometry. US Patent No. 4655225 discloses a spectrophotometric method for the non-invasive determination of glucose concentration in body tissue. It involves shining a beam of light from a directional light source onto a selected body part and collecting the resulting radiation emanating from that body part. The collected radiation includes at least one band with wavelengths of 1575nm, 1756nm, 2100nm and 2270±15nm, which is a typical absorption spectrum of glucose and can be used for...

AI Technical Summary

Problems solved by technology

[0005] However, due to the strong absorption of pure water in the near-infrared, the difference between the characteristic peak of glucose and the absorption peak of water is small. It is not easy to find the characteristic absorption peak of glucose directly from the near-infrared spectrum of the glucose aqueous solution collected, and it is impossible to quickly and accurately identify it.

Therefore, the application of near-infrared spectroscopy to the detection of glucose in aqueous solutions has not been reported in the literature.

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