Compound pesticide of tebuconazole and ningnan mycin
A technology of Ningnanmycin and tebuconazole, applied in the field of pesticides
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Embodiment 1
[0045] Embodiment 1 The preparation of the composition containing Ningnanmycin
[0046] Inoculate the frozen Streptomyces noursii var. Xichang (purchased from Heilongjiang Biopesticide Engineering Technology Research Center) on a freshly prepared solid medium, at 28 ° C, 80% relative humidity, and under the condition of sterile air circulation Cultured for 16 days for rejuvenation, and the resulting strain spores were picked. Then produce mycelium, that is, to inoculate the spores of the strain on the freshly prepared solid medium, and culture it aerobically at 28°C, 80% relative humidity, and under the condition of sterile air circulation for 14 days, and the mycelium can be clearly seen produce. Carry out proliferation culture then, promptly use the first freshly prepared liquid medium to wash mycelia from the surface of the solid medium and enter a 1L Erlenmeyer flask, add the first freshly prepared liquid medium to supplement to a medium volume of 250ml, Incubate for 14 ...
Embodiment 2
[0050] The formula of embodiment 2 compound pesticides
[0051]1, 50% tebuconazole + 5% Ningnanmycin wettable powder (WP): get tebuconazole 50g; Ningnanmycin 4g prepared in Example 1; sodium lauryl sulfate (wetting agent) 2g Sodium lignosulfonate (dispersant) 4g; Sodium methylene dinaphthalene sulfonate (NN0, dispersant) 5g; Kaolin (filler) was added to 100g, and ground into wettable powder.
[0052] 2. 28% tebuconazole+2% Ningnanmycin suspension concentrate (SC): 28 g of tebuconazole; 2 g of Ningnanmycin prepared in Example 1; 3 g of sodium lignosulfonate (dispersant); methylene Sodium dinaphthalenesulfonate (NNO, dispersant) 2g; ethylene glycol (antifreeze) 4g; xanthan gum (thickener) 0.2g; silicone oil (defoamer) 0.3g; deionized water to make up to 100g.
[0053] 3. 10% tebuconazole+2% Ningnanmycin microemulsion (ME): get tebuconazole 10g; Ningnanmycin 2g prepared in Example 1; dichloromethane (solvent) 15g; dodecyl Calcium benzenesulfonate (emulsifier) 4g; phenylethylp...
Embodiment 3
[0056] The control effect of embodiment 3 compound pesticides
[0057] We dilute the 28% tebuconazole+2% Ningnanmycin suspension concentrate (SC1) prepared according to Example 2 with an equivalent amount of commercially available 30% tebuconazole suspension concentrate (purchased from Jiangsu Kesheng Group Co., Ltd. ) (SC2) and the 28% tebuconazole+2% Ningnanmycin suspension concentrate (SC3) prepared with the original drug (SC3; it refers to the corresponding formula of Example 2, but with the original drug of Ningnanmycin (purchased from Heilongjiang Qianger Biochemical Technology Development Co., Ltd.) instead) to do field experiments to show its control effect on various crops.
[0058] In the high-incidence area of Henan Province, the 2-mu rice test field adjacent to the fish pond was equally divided, and each area was tested with SC1, SC2, SC3 and blank control (CP), and the rice planted in it was fertilized in the same amount in late July. The medicine was applied o...
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