Agaricus bisporus polysaccharide with immunological activity as well as preparation method and application of agaricus bisporus polysaccharide

A technology of Agaricus bisporus and immune activity, which is applied in the field of Agaricus bisporus polysaccharide and its preparation, and can solve the problems of the source of activity, the relationship between activity and structure, but the progress is slow.

Inactive Publication Date: 2011-05-25
曹庸
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The health benefits of Agaricus bisporus have been confirmed by a long history of consumptio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1), the preparation of Agaricus bisporus polysaccharide:

[0019] (a) Prepare 1000g of Agaricus bisporus, dry and pulverize at 45°C, add 3000g of distilled water, extract in a water bath at 75°C for 2 hours, filter, and concentrate in vacuo;

[0020] (b) Precipitate with 80% ethanol, centrifuge (4500r, 15min) and discard the supernatant;

[0021] (e) Segva deproteinization 6 times;

[0022] (f) select ion-exchange filler (D201, DEAE-52, CM52) to carry out ion-exchange column chromatography to obtain ABPI;

[0023] (g) Gel column packing (Sephadex G-200, Bio-Gel-p-4, sepharose 4B, sephacryl S100, sephacryl S300) was selected for gel column chromatography to obtain 1000 mg of polysaccharide ABPIa.

[0024] (2) Analysis method of polysaccharide structure

[0025] (1) Determination of the molecular weight range of Agaricus bisporus polysaccharide ABPIa with high pressure gel exclusion chromatography (HPGPC);

[0026] (2) The glycosidic bond structure is analyzed by per...

Embodiment 2

[0048] The preparation method of Agaricus bisporus polysaccharide, concrete steps are:

[0049] (a) Prepare 2000g of Agaricus bisporus, dry and pulverize at 50°C, extract in water bath at 80°C for 4 hours, filter and concentrate;

[0050] (b) Precipitate with 80% ethanol, centrifuge (500or, 10min) and discard the supernatant;

[0051](c) Deproteinize 4 times by Segva method to obtain crude polysaccharide ABP;

[0052] (d) select ion-exchange filler (D202, CM32, CM52) to carry out ion-exchange column chromatography;

[0053] (e) Gel packing (Sephadex G-100, Bio-Gel-p-2, sepharose 2B, sephacryl S100) was selected for gel column chromatography separation to obtain 1500 mg Agaricus bisporus polysaccharide ABPIa

Embodiment 3

[0055] The preparation method of Agaricus bisporus polysaccharide, concrete steps are:

[0056] (a) Prepare 500 g of Agaricus bisporus, dry and pulverize at 55°C, extract in a water bath at 65°C for 2 hours, filter and concentrate;

[0057] (d) Precipitate with 95% ethanol, centrifuge (5000r, 25min), discard the supernatant;

[0058] (e) Deproteinize 8 times by Segva method to obtain the crude polysaccharide ABP of Agaricus bisporus;

[0059] (f) Selecting ion-exchange fillers (D201, D202, CM32, CM52) to perform ion-exchange column chromatography to obtain Agaricus bisporus polysaccharide ABPI.

[0060] (g) (Sephadex G-200, sepharose 2B, sepharose 4B, sephacryl S300) were subjected to gel column chromatography to obtain 500 mg of Agaricus bisporus polysaccharide ABPIa.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to agaricus bisporus polysaccharide with immunological activity. The agaricus bisporus polysaccharide is heteropolysaccharide consisting of ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose, wherein the mole mass ratio of ribose to rhamnose to arabinose to xylose to mannose to glucose to galactose is 2.08:4.61:2.45:22.25:36.45:89.22:1.55. The preparation method comprises the following steps: carrying out water bath on agaricus bisporus; carrying out ethanol precipitation on extract with ethanol; carrying out deproteinization on ethanol sediments by using a Sevag method; performing ion exchange column chromatography on the obtained crude polysaccharose anion exchange padding; eluting with distilled water, and freeze-drying; and carrying out gel chromatography, eluting with distilled water, and freeze-drying, so as to obtain the agaricus bisporus polysaccharide. The polysaccharide has the characteristic of immunologic enhancement, can be used as an adjuvant therapy medicament for hypoimmunity diseases such as tumour or used as functional health care products and foods which have the effect of improving the immunity.

Description

technical field [0001] The present invention relates to an immune active polysaccharide, in particular to a type of immune active Agaricus bisporus polysaccharide and its preparation method and application. technical background [0002] Agaricus bisporus is flat in nature and sweet in taste, can invigorate the spleen and qi, and can enhance the body's ability to resist diseases. It has a long history of consumption. It is the edible fungus with the most extensive cultivation, the highest output and the largest consumption in the world. It is also the product that earns the most foreign exchange from the export of edible fungi in my country. According to statistics from the China Edible Fungi Association, the output of Agaricus bisporus in my country reached 20 million tons in 2009, mainly exported as processed products such as canned mushrooms, dried mushroom slices, and salted mushrooms. A polysaccharide (PS-K) can be extracted from mushrooms, which has anti-virus and ant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/02A23L1/28A23L1/09A23L31/00A23L33/125
Inventor 曹庸叶婧陈雪香刘欣
Owner 曹庸
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap