Compound microbial agent for treating dye waste water as well as preparation and application of compound microbial agent

A technology of composite microbial agent and dye wastewater, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of large limitations in decolorization and degradation, inability to adapt to changes in dye types, water quality fluctuations, and inability to use multiple dyes Discoloration and other problems, to achieve the effect of low cost, high adaptability, and easy compounding

Inactive Publication Date: 2011-06-01
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for effective use of an organism called Bacillus spores (Bacillusssp.) when treating different types of industrial or commercial wastes such as those containing dichroinsulfonate dyestuffs. It also includes various methods used during manufacturing processes to make it more efficient at decompositing these chemical substances into harmless materials like nitrosothiazole sulfonic ammoniums). Overall, this new biological material provides technical benefits including improved ability to remove colored contaminants from aqueous solutions without causing environmental harm while maintaining their original properties.

Problems solved by technology

This patents discusses different ways for treating or removing harmful substances from dyed materials like paper pulp during manufacturing processes. Chemistry method requires expensive equipment that may be limited with regards to changing conditions over time and does little work at all if there are many other compounds found within them. Biology techniques have shown promise but they also face challenges when dealing with various colored agents used in these industries.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] Acinetobacter haemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans were respectively cultured in beef extract peptone medium LB for 18-24 hours, The culture temperature is 20-37°C, the pH of the culture medium is 6.0-8.0, and the OD 600 0.6±0.05 activation solution of Acinetobacter hemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter lutherii, Klebsiella nigerii; The activation solution of Helicobacter, Bacillus flexus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans are respectively 12%: 12%: 12%: 24%: 20%: 20% by volume Mix to obtain the desired compound microbial agent.

[0030] 1. It is used to treat reactive red dye wastewater. The initial dye concentration is 60mg / L, and the chromaticity and absorbance value represent 0.952. The dye reactive red 195 is an azo dye with a complex structure, and it is very difficult to treat it with a single microbial age...

Embodiment 2

[0034] Acinetobacter haemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans were respectively cultured in beef extract peptone medium LB for 18-24 hours, The culture temperature is 20-37°C, the pH of the culture medium is 7.0-7.2, and the OD 600 0.6±0.05 activation solution of Acinetobacter hemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter lutherii, Klebsiella nigerii; The activating solution of Helicobacter, Bacillus flexus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans is mixed by volume percentage 14%: 28%: 21%: 7%: 17.5%: 12.5%. Obtain the desired compound microbial bacterial agent.

[0035] 1. Take 1000mL of reactive red 195 dye wastewater with an initial dye concentration of 60.00mg / L and a chromaticity absorbance of 0.952, add glucose 1.5g, sodium chloride 1g, potassium dihydrogen phosphate 2.65g, disodium hydrogen phosphate 4.26g, and MgSO 4 ·7H 2 O 0...

Embodiment 3

[0038] Acinetobacter haemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans were respectively cultured in beef extract peptone medium LB for 18-24 hours, The culture temperature is 20-37°C, the pH of the culture medium is 7.0-7.2, and the OD 600 0.6±0.05 activation solution of Acinetobacter hemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter lutherii, Klebsiella nigerii; The activating solution of Helicobacter, Bacillus flexus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans is mixed by volume percentage 28%: 14%: 21%: 7%: 17.5%: 12.5%. Obtain the desired compound microbial bacterial agent.

[0039] 1. Take 1000 mL of malachite green dye wastewater with an initial dye concentration of 80 mg / L and a chromaticity absorbance value of 1.629, add 1.5 g of glucose, 1 g of sodium chloride, 2.65 g of potassium dihydrogen phosphate, and 4.26 g of disodium hydrogen phosphate,...

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Abstract

The invention discloses a compound microbial agent for treating dye waste water as well as preparation and application of the compound microbial agent. The compound microbial agent is formed by complexing acinetobacter haemolyticus, Escherichia coli, bacillus flexus, Yersinia intermedia, luther enterobacter and Klebsiella pneumoniae. Complexing is simple and convenient, the broad spectrum is strong, the difficulty that a single microbial agent can not meet the requirement of water quality variation is overcome, and the cost of treating the dye industrial waste water is reduced.

Description

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Claims

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Application Information

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Owner HEFEI UNIV OF TECH
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