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Preparation method of organic selenium enzyme preparation

An enzyme preparation and organic selenium technology, applied in the field of preparation of organic selenium enzyme preparations, can solve the problems of affecting the use effect and low conversion rate

Inactive Publication Date: 2011-06-08
滨州市正元畜牧发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this preparation method usually only uses one kind of bacterium to carry out one conversion, and the conversion rate of inorganic selenium into organic selenium is low, only 30%~60%, affects the use effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] A preparation method of an organic selenium enzyme preparation, the method is, according to 81 parts by weight of bran, 13 parts by weight of soybean meal, 0.9 parts by weight of rice husk powder, 0.6 parts by weight of ammonium sulfate, 0.4 parts by weight of potassium dihydrogen phosphate, malt 4 parts by weight, the proportioning of 110 parts by weight of sodium selenite solution with a mass percentage concentration of 0.1% is mixed with the above materials to obtain a mixture, after stirring evenly, when the water content of the mixture is insufficient, supplement the mixture with sterile water water, so that its water content is 65% by weight, and then, under the condition of pressure of 0.18MPa and temperature of 125°C, carry out high-temperature and high-pressure sterilization for 43 minutes. After sterilization, when the above-mentioned materials are cooled to 38°C , inoculate the aspergillus classification of 2.3% of its weight Candida utilis (Candida utilis), c...

Embodiment 2

[0013] A preparation method of an organic selenium enzyme preparation, the method is as follows: 85 parts by weight of bran, 11 parts by weight of soybean meal, 1.1 parts by weight of rice husk powder, 0.4 parts by weight of ammonium sulfate, 0.6 parts by weight of potassium dihydrogen phosphate, malt 2 parts by weight, the proportioning of 90 parts by weight of sodium selenite solution with a concentration of 0.2% by weight. Mix the above materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, supplement the mixture with sterile water. water, so that its water content is 55% by weight, and then, under the condition of pressure of 0.22MPa and temperature of 115°C, carry out high-temperature and high-pressure sterilization for 46 minutes. After the sterilization, when the above-mentioned materials are cooled to 36°C , inoculate the aspergillus classification of 2.7% of its weight Candida utilis (Candida utilis), cultivate 33 h...

Embodiment 3

[0015] A method for preparing an organic selenium enzyme preparation, the method is as follows: 83 parts by weight of bran, 12 parts by weight of soybean meal, 1.0 part by weight of rice husk powder, 0.5 part by weight of ammonium sulfate, 0.5 part by weight of potassium dihydrogen phosphate, malt 3 parts by weight, the ratio of 100 parts by weight of sodium selenite solution with a concentration of 0.15% by mixing the above materials to obtain a mixture, after stirring evenly, when the water content of the mixture is insufficient, supplement the mixture with sterile water water, so that its water content is 60% by weight, and then, under the condition of pressure of 0.20MPa and temperature of 120°C, carry out high temperature and high pressure sterilization for 45 minutes. After sterilization, when the above materials are cooled to 37°C , inoculate the aspergillus classification of the 2.5% Candida utilis (Candida utilis) of its weight, stir and cultivate at 28 ℃ of temperatur...

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Abstract

The invention provides a preparation method of an organic selenium enzyme preparation, comprising the following steps of: mixing bran, bean pulp, rice hull powder, a sodium selenite solution, and the like, sterilizing at high temperature and high pressure, inoculating a kojitray strain of candida utilis after sterilization, culturing and then taking out a material obtained through culturing, inoculating a kojitray strain of aspergillus usamii on the material obtained through culturing, culturing for the second time, inoculating a kojitray strain of bacillus subtilis on a material obtained through culturing for the second time, culturing for the third time, drying and crushing a material obtained through culturing for the third time to prepare the organic selenium enzyme preparation. In the preparation method of the organic selenium enzyme preparation, continuous three times of conventions are carried out by adopting three types of strains, the conversion ratio of converting original inorganic selenium with extremely low valence into organic selenium with higher valence can reach above 98 percent, and the prepared enzyme preparation integrates organic trace elements, enzyme preparation and probiotic bacteria into a whole, simultaneously has a plurality of biological functions and has the efficacy of reducing anti-nutritional factors.

Description

technical field [0001] The invention provides a preparation method of an organic selenium enzyme preparation. Background technique [0002] At present, most of the selenium products sold in the feed additive market are inorganic selenium, that is, sodium selenite, which has low potency when used, and long-term use will pollute water quality and the environment. Although there is a part of organic selenium, most of them are industrially synthesized products with high cost and expensive price. Now there is a preparation method of an organic selenium enzyme preparation, which uses probiotics such as fungi and bacteria as carriers to convert inorganic selenium into organic selenium through biotransformation, thereby improving the potency and avoiding the pollution caused by the use of inorganic selenium. However, this preparation method usually only uses one kind of bacteria for one conversion, and the conversion rate of inorganic selenium into organic selenium is low, only 30%...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K10/12A23K20/189A23K20/20
Inventor 吴希恩
Owner 滨州市正元畜牧发展有限公司
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