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Attenuated live vaccine and application thereof

A technology of attenuated live vaccines and preservation numbers, which is applied in the field of vaccines, can solve the problems of exacerbation of infection conditions, and achieve the effect of convenient preparation and good protective effect

Inactive Publication Date: 2012-07-04
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no effective prevention and control measures due to the risk of exacerbating infectious conditions due to antibiotic treatment

Method used

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  • Attenuated live vaccine and application thereof
  • Attenuated live vaccine and application thereof
  • Attenuated live vaccine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Immune response stimulated by enterohemorrhagic Escherichia coli O157:H7 strain 00B015 (the deposit number is CCTCC M2010185)

[0015] 1 Experimental method

[0016] 1) Isolation and identification of enterohemorrhagic Escherichia coli O157:H7 strain 00B015:

[0017] Enterohaemorrhagic Escherichia coli O157:H7 strain 00B015 was isolated from a patient with common diarrhea in Jiangsu Center for Disease Control and Prevention. China Center for Type Culture Collection, the collection number is CCTCC M 2010185.

[0018] 2) Divide 20 6-week-old Balb / c mice on average into 2 groups, 10 in each group. Before oral inoculation, the mice in each group were fasted overnight and deprived of water for 4 hours. First give 300ul of 3% sodium bicarbonate solution orally to neutralize gastric acid and prevent the damage of gastric acid to the bacterial strain. After 20min, take 300ul of the above-mentioned prepared bacterial solution and feed each group of mice (about 1×10...

Embodiment 2

[0031] Example 2 Immune protection identification of enterohemorrhagic Escherichia coli O157:H7 strain 00B015

[0032] 1 Experimental method

[0033] 10 days after the third immunization, the mice were given a sterile aqueous solution containing 5g / L streptomycin to drink for 3 days, and then 1×10 9 CFU / dose of O157-SM R 2 (Cheng Jianping, Yi Yong, Mao Xuhu, Zou Quanming. Establishment of animal model of Escherichia coli O157:H7 infection [J]. Journal of Microbiology, 2004, (5).), the whole bacterial solution was divided into two times, with an interval of 6h, and administered orally After entering the gastrointestinal tract of mice, closely observe the changes of the activity, food intake and mental state of the mice after the challenge, calculate the number of deaths of the mice at the end of the 28-day observation period, and take samples 3, 7, 14, and 3 days after the challenge. On the 28th day, feces were counted, resuspended in sterile normal saline, crushed with a ...

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Abstract

The invention provides an attenuated live vaccine and an application thereof. The collection number of the attenuated live vaccine is CCTCC M2010185. The attenuated live vaccine is a nontoxic enterohemorrhagic escherichia coli O157: H7 strain, which contains enterohemorrhagic escherichia coli O157: H7 antigens. The attenuated live vaccine can be used for preparing preparations for preventing enterohemorrhagic escherichia coli O157: H7 infection. The strain of the attenuated live vaccine is convenient to prepare, has all the antigens of the enterohemorrhagic escherichia coli O157: H7, can stimulate the systemic anti-enterohemorrhagic escherichia coli O157: H7 immunoreaction and further has a good protective effect.

Description

technical field [0001] The invention relates to a vaccine, in particular to an attenuated live vaccine and its application. Background technique [0002] Enterohemorrhangic E.coli (EHEC) O 157:H7 (EHECO157) is an important pathogen of zoonotic infectious diseases, and its infection has become a global public health problem. Since the bacterium was first identified as a serious pathogen in 1982, many countries and regions around the world, including China, have experienced multiple outbreaks. EHEC O157 infection of the host can lead to diarrhea, hemorrhagic enteritis, hemolytic uremic syndrome and other diseases and serious complications. Because antibiotic treatment can exacerbate the risk of infectious conditions, there are currently no effective prevention and control measures. EHEC O157 is easy to cultivate, highly infective, and has various transmission routes, making it very likely to be used as a bacterial weapon and bioterrorism agent in future military struggles. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02
Inventor 顾江朱凤才毛旭虎邹全明
Owner ARMY MEDICAL UNIV