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Preparation method of plant immune protein

A technology of plant immune protein and bacteria, which is applied in the field of preparation technology of plant immune protein, to achieve the effect of good quality, high process efficiency, safe and pollution-free preparation process

Active Publication Date: 2013-06-05
北京东方先科科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the NAC protein with plant immune function, how to prepare and produce it will directly affect the industrial application of NAC protein, but the current research focuses on the mechanism of the protein, and there is no research on the mass production and preparation process of NAC protein. related reports

Method used

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  • Preparation method of plant immune protein
  • Preparation method of plant immune protein
  • Preparation method of plant immune protein

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Experimental program
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Embodiment

[0030] As if figure 2 The preparation technology of the plant immunogen protein of the present invention specifically includes the following steps:

[0031] 1. Fermented after fermentation ( Pichia Fermentans ) Cultivation of strains

[0032] CGMCC 2.1838 (not limited to the strain, such as the strain of the trunk, such as the strain of the trunk) strain is cultivated with a PDA medium test tube, and then the 50%liquid paraffin oil is sealed and placed in a ultra-low temperature refrigerator at -70 ° C.A large amount of bacteria are cultivated with PDA liquid medium.

[0033] On the clean workbench after the ultraviolet light irradiation 25min, first pick a small amount of fermentation from the low -temperature storage pipe to be fermented CGMCC 2.1838 bacteria, transfer the bacteria to the solid PDA medium, and then place it at 37 ° CCultivate 24h in the constant temperature culture box, complete the first -stage fermentation, then pick a small amount of bacteria from the solid P...

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Abstract

The invention discloses a preparation method of a plant immune protein. The method comprises the following steps: picking a small amount of bacteria from a Pichia pastoris tube and transferring to a solid potato dextrose agar (PDA) culture medium, cultivating for at least 24 hours in constant temperature incubator at a 28-37 DEG C; then picking a small amount of bacteria from the solid PDA culture medium to inoculate in liquid PDA culture medium, performing shaking cultivation at 37 DEG C under 180-200rpm for at least 24 hours; cutting the cultivated bacteria to break the cells of the bacteria and obtain crude protein solution; adding ammonium sulfate in the crude protein solution to ensure that the final concentration reaches 20%, centrifuging the solution to collect precipitate; and dissolving the obtained precipitate again to obtain protein solution, adding the protein solution in carrier, mixing evenly, and performing spray drying to prepare the plant immune protein. The preparation process disclosed by the invention has the advantages of low cost, high yield and good product quality, and is simple in operations.

Description

Technical field [0001] The present invention involves a protein preparation, especially the preparation process of plant immune protein. Background technique [0002] Nascent Polypeptide-Associated Complex was first purified by intact purification. It was also the earliest report that the protein could recognize the signal granules SRP (Signal Recognition).The synthetic polypeptide.Two new peptide composite proteins (referred to as NAC protein) were obtained through affinity purification, named α-nac protein and β-nac protein, respectively.Previously, according to the results of the SDS-PAGE electrophoresis, the molecular weights of the two proteins were initially determined that the two proteins were 33kd and 22kd, but the real molecular weight of the protein was only 23.492kd and 17.352kd until 2000.The CDNA sequence of the NAC protein gene is shown in SEQ ID NO: 1, which is named Zmnac1 protein (Genbank login number EU224278). The gene sequence is 1029bp in length and has a co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C07K1/30A01N63/04A01P1/00A01P3/00C12R1/84
Inventor 符江华
Owner 北京东方先科科技有限公司
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