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Immobilized cell bioreactor

A technology for bioreactors and immobilized cells, applied in solid-phase fermentation bioreactors, immobilized enzymes, biochemical equipment and methods, etc., can solve the problems of high cost, achieve low liquid shear force, wide sources, and easy Magnified effect

Active Publication Date: 2011-07-20
WUHAN CEKG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Membrane-type immobilized cell bioreactor has high catalytic efficiency, low operating pressure drop, easy to control fluid dynamics, easy to scale up, and provides more uniform environmental conditions. At the same time, it can also separate products in time to relieve feedback inhibition of products , the downstream processing is simple, and it is the most widely used bioreactor in continuous large-scale production, but the cost of building this reactor is relatively high

Method used

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  • Immobilized cell bioreactor
  • Immobilized cell bioreactor
  • Immobilized cell bioreactor

Examples

Experimental program
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Embodiment 1

[0054] Example one: such as Image 6 As shown, the design of the present invention is a new type of bioreactor modified on the basis of a traditional fermenter, which can be used for cell immobilization. The whole reactor is cylindrical with inner diameter d 2 =240mm, height H=360mm, diameter-to-height ratio is 1:1.5, and the actual usable volume of the reactor is 15L. Chassis diameter d 1 =200mm, d 1 / d 2 =1:1.2, height h 2 =10mm, fixed on the bottom center of the reactor by welding or screws, 0.9d from the top edge of the pan to the center of the chassis 1 There are four small holes that can be installed with baffle symmetrically distributed, the depth of the small holes is h 1 And chassis height h 2 The ratio is 0.9. There is a cylindrical space in the center of the chassis with a diameter d 3 =50mm, d 3 / d 1 0.25, depth h 3 =10mm, h 3 / h 2 =1.0, the stirring shaft base is installed in the center of the space, and its height is h 4 =8mm, h 4 / h 3 =0.8, the vent pipe ring-shap...

Embodiment 2

[0055] Example 2: The immobilized cell bioreactor designed in the present invention can be used for the enzymatic enrichment of glyceride DHA and EPA in crude fish oil. First, it is necessary to explore the growth and enzyme production conditions of the cells to be fixed and optimize the composition of the fermentation medium and fermentation conditions. The object to be immobilized selected in this example is Candida columnar. Such as Figure 5 As shown, without installing the isolation cylinder, the carrier, and the carrier protective cover, the reactor is used for free cylindrical Candida cell fermentation by installing four baffles at the corresponding positions of the chassis.

[0056] When used for fermentation of immobilized Candida columnar cells, the reactor is assembled in the following order:

[0057] (1) Install the stirring paddle and the defoaming rake on the stirring shaft in turn, and then fix the stirring shaft to the base in the central space of the chassis;

[00...

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Abstract

The invention relates to an immobilized cell bioreactor, in particular to an immobilized cell bioreactor capable of implementing continuous production and application thereof in continuous enzymolysis of biological active substances. The immobilized cell bioreactor mainly comprises a reaction vessel and a carrier arranged in the reaction vessel, wherein immobilized cells are carried by the carrier, and a feed solution is subjected to enzymolysis of the immobilized cells in the carrier so as to generate a target product. The immobilized cell bioreactor is characterized in that the carrier is asingle hollow cylindrical block made of porous materials, the feed solution is injected into the hollow part and is subjected to enzymolysis of the immobilized cells in the carrier in the carrier permeation process so as to generate the target product, and the target product is discharged out of the vessel. The invention belongs to an immobilized cell bioreactor capable of implementing continuousproduction.

Description

Technical field [0001] The invention relates to an immobilized cell bioreactor, in particular to an immobilized cell bioreactor capable of continuous production, and its application in continuous enzymatic hydrolysis of biologically active substances. Background technique [0002] Immobilization technology refers to the use of various physical and chemical methods to confine free cells or enzymes in specific spatial areas, increase the concentration of cells or enzymes, and maintain biological activity and reuse a technology. Cells or enzymes made using this technique are called immobilized cells or immobilized enzymes. [0003] Enzyme technology is often applied to the hydrolysis of biological macromolecules to prepare various small molecular substances with physiological activities. However, after the reaction, the free enzyme is mixed in the product to increase the difficulty of product separation, and the free enzyme is directly exposed to the environment, and its catalytic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/16C12N11/00C12P7/64
Inventor 罗红宇滕宏飞王斌何健东王朋辛建美
Owner WUHAN CEKG TECH CO LTD