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Immobilized cell bioreactor

A bioreactor, immobilized cell technology, applied in solid-phase fermentation bioreactors, immobilized enzymes, biochemical equipment and methods, etc., can solve the problem of high cost, achieve low liquid shear force, low mass transfer resistance , easy to enlarge effect

Active Publication Date: 2013-04-17
WUHAN CEKG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Membrane-type immobilized cell bioreactor has high catalytic efficiency, low operating pressure drop, easy to control fluid dynamics, easy to scale up, and provides more uniform environmental conditions. At the same time, it can also separate products in time to relieve feedback inhibition of products , the downstream processing is simple, and it is the most widely used bioreactor in continuous large-scale production, but the cost of building this reactor is relatively high

Method used

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  • Immobilized cell bioreactor
  • Immobilized cell bioreactor
  • Immobilized cell bioreactor

Examples

Experimental program
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Embodiment 1

[0054] Embodiment one: if Image 6 As shown, what the present invention designs is a kind of novel bioreactor that can be used for cell immobilization, which is transformed on the basis of traditional fermenter. The reactor is cylindrical as a whole, with an inner diameter d 2 =240mm, height H=360mm, ratio of diameter to height is 1:1.5, and the actual usable volume of the reactor is 15L. Chassis diameter d 1 =200mm, d 1 / d 2 =1:1.2, height h 2 =10mm, fixed at the center of the bottom of the reactor by welding or screws, the upper edge of the plate, 0.9d from the center of the chassis 1 There are four small holes that are symmetrically distributed to install the baffle, and the depth of the small holes is h 1 and chassis height h 2 The ratio is 0.9. There is a cylindrical space in the center of the chassis with a diameter d 3 =50mm, d 3 / d 1 is 0.25, the depth h 3 =10mm, h 3 / h 2 =1.0, the stirring shaft base is installed in the center of the space, its height h ...

Embodiment 2

[0055] Example 2: The immobilized cell bioreactor designed in the present invention can be used for enzymatic enrichment of glyceride-type DHA and EPA in crude fish oil. First of all, it is necessary to explore the growth and enzyme production conditions of the cells to be fixed and optimize the components of the fermentation medium and fermentation conditions. The object to be immobilized in this example is Candida cylindrica. Such as Figure 5 As shown, the reactor is used for the fermentation of free Candida columnar cells by installing four baffles at the corresponding positions of the chassis without installing the isolation cylinder, carrier, and carrier protective cover.

[0056] For the fermentation of immobilized Candida columnar cells, assemble the reactor in the following order:

[0057] (1) Install the stirring paddle and defoaming rake on the stirring shaft in turn, and then fix the stirring shaft on the base located in the central space of the chassis;

[0058...

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Abstract

The invention relates to an immobilized cell bioreactor, in particular to an immobilized cell bioreactor capable of implementing continuous production and application thereof in continuous enzymolysis of biological active substances. The immobilized cell bioreactor mainly comprises a reaction vessel and a carrier arranged in the reaction vessel, wherein immobilized cells are carried by the carrier, and a feed solution is subjected to enzymolysis of the immobilized cells in the carrier so as to generate a target product. The immobilized cell bioreactor is characterized in that the carrier is asingle hollow cylindrical block made of porous materials, the feed solution is injected into the hollow part and is subjected to enzymolysis of the immobilized cells in the carrier in the carrier permeation process so as to generate the target product, and the target product is discharged out of the vessel. The invention belongs to an immobilized cell bioreactor capable of implementing continuousproduction.

Description

technical field [0001] The invention relates to an immobilized cell bioreactor, in particular to an immobilized cell bioreactor capable of continuous production, and its application in continuous enzymatic hydrolysis of biologically active substances. Background technique [0002] Immobilization technology refers to the use of various physical and chemical methods to bind free cells or enzymes in a specific space area, increase the concentration of cells or enzymes, and maintain biological activity and repeated use. The cells or enzymes made by this technique are called immobilized cells or immobilized enzymes. [0003] Enzyme technology is often applied to the hydrolysis of biological macromolecules to prepare various small molecular substances with physiological activities. However, after the reaction, the free enzyme is mixed in the product, which increases the difficulty of product separation, and the free enzyme is directly exposed to the environment, and its catalytic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/16C12N11/00C12P7/64
Inventor 罗红宇滕宏飞王斌何健东王朋辛建美
Owner WUHAN CEKG TECH CO LTD