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Production method of natural red pigment

A technology of natural red pigment and production method, which is applied in the field of fermentation to produce target objects, and can solve the problems of no red pigment, high extraction cost, difficult industrial production, and low yield.

Inactive Publication Date: 2011-08-03
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are reports at home and abroad on the use of bacteria and rhodotorula to ferment and produce red pigment, but due to the low yield of the selected strains and the high extraction cost, it is difficult to industrialize production
The deep red pigment produced by Penicillium var. armeniaca in the genus Penicillium has been commercially developed and produced by Ascolor Biotech in the Czech Republic, but so far there has been no report on the production of red pigment by dual fermentation of Penicillium and yeast

Method used

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  • Production method of natural red pigment
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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The effect of different inoculation ratios of Penicillium New Zealand and Candida tropicalis on the production of red pigment

[0014] After activation of Penicillium New Zealand and Candida tropicalis according to the above-mentioned "strain activation" step, they were respectively inoculated into the liquid fermentation medium at 30°C, 150rpm, and cultured on a shaker for 1 day to prepare a bacterial suspension. Then, the two bacterial suspensions were inoculated into the 151 fermentor with different inoculation ratios (3:1; 2:1; 1:1; 1:2; 1:3), the total inoculation amount was 10%, and the aerated culture, The speed of the stirrer is about 600rpm, the temperature is controlled at 30°C, and the pH is controlled at about 6, put into the tank after 48 hours, and the pigment output is shown in Table 1:

[0015] Table 1 The effect of different inoculation ratios on pigment production

[0016]

Embodiment 2

[0017] Example 2 The influence of different culture temperatures on the production of red pigment

[0018] After activation of Penicillium New Zealand and Candida tropicalis according to the above-mentioned "strain activation" step, they were respectively inoculated into the liquid fermentation medium at 30°C, 150rpm, and cultured on a shaker for 1 day to prepare a bacterial suspension. Then, an equal volume of the two bacteria suspension was inoculated into the 151 fermentor, the total inoculation amount was 10%, aerated culture, the stirrer speed was about 600rpm, and the temperature was controlled at 26℃, 28℃, 30℃, 32℃, At 34°C, 36°C, the pH is controlled at about 6, and the cans are placed after 48 hours. The pigment output is shown in Table 2:

[0019] Table 2 The influence of different temperatures on the yield of pigments

[0020]

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Abstract

The invention discloses a production method of natural red pigment, and relates to a fermentation produced target substance. The invention aims to provide a natural red pigment production method with simple process, high yield and low cost. The invention has the technical scheme that the natural red pigment production method uses New Zealand penicillium and candida tropicalis, and comprises the steps of strain activation and culture medium culturing; wherein the volume ratio of the New Zealand penicillium suspension to candida tropicalis suspension is 1-3:3-1, and the culturing temperature is 26-36 DEG C. The invention provides the production method of the natural red pigment.

Description

Technical field: [0001] The invention relates to a fermentation production target, in particular a production method of natural red pigment. Background technique: [0002] Pigments are widely used in food, cosmetics and other fields. There are two types of artificial and natural pigments. Artificially synthesized pigments have been banned or restricted due to their varying degrees of toxicity. Natural pigments have attracted more and more attention. At present, there are two methods for producing natural pigments, one is to extract from plants, but the cost is high; the other is to produce by microbial method, which has the advantages of simple process, production is not restricted by site and season, and the equipment used is simple. At present, there are reports at home and abroad about using bacteria and red yeast to ferment to produce red pigments, but due to the low yield of selected strains and high extraction costs, it is difficult to industrially produce. The dark red p...

Claims

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Application Information

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IPC IPC(8): C12P7/26
Inventor 王海磊
Owner HENAN NORMAL UNIV